Procurou por: RT-PCR and cDNA Reagents

Código de Artigo: (71978-3)

Fornecedor: Merck Millipore (Novagen)

Descrição: The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots,<i> in situ</i> hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant <i>Thermus thermophilus</i> (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.

UOM: 1 * 1 KIT

Promoção

Código de Artigo: (27-9264-01)

Fornecedor: Cytiva

Descrição: Ready-To-Go™ You-Prime First-Strand beads are preformulated, single-dose reaction beads prepackaged in thin-walled 0,5 ml tubes compatible with most thermal cyclers. Ready-To-Go™ You-Prime First-Strand beads are used for synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice.

UOM: 1 * 50 Tests

MSDS Promoção

Código de Artigo: (27-9261-01)

Fornecedor: Cytiva

Descrição: Designed to generate full length first-strand cDNA from mRNA templates. Following first-strand cDNA synthesis, the sample can be used directly for <i>in vitro</i> amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method.

UOM: 1 * 55 Tests

Promoção

Fornecedor: Cytiva

Descrição: Pre-dispensed, freeze-dried beads that include the reagents necessary for one-step reverse transcription-PCR with high sensitivity and reproducibility.

Fornecedor: Promega

Descrição: The ImProm-II™ reverse transcription system produces efficient, robust synthesis of first-strand cDNA in preparation for PCR amplification. The components can be used to reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA. The optimised reaction buffer and ImProm-II™ Reverse Transcriptase provided in the system together enable robust, full-length cDNA synthesis for the reproducible analysis of rare or long messages. The cDNA synthesis conditions were formulated for standalone applications or for easy transition to gene-specific target amplification. An aliquot of the reverse transcription reaction (1 to 20 μl) can be amplified directly using Taq DNA polymerase in coupled or uncoupled PCR.

Fornecedor: Promega

Descrição: The Reverse Transcription system provides reagents to efficiently reverse transcribe RNA into cDNA. The cDNA prepared from each reaction using this system may be used directly in multiple PCR amplifications using Taq DNA polymerase. The AMV Reverse Transcriptase synthesises single-stranded cDNA from total or poly(A)+ RNA. Both Oligo(dT)15 and random primers are included, allowing cDNA synthesis from virtually any RNA source. The system contains sufficient reagents for 100 cDNA synthesis reactions, processing 1 μg of RNA per reaction. Each cDNA synthesis reaction may be divided and used in up to 20 separate PCR amplifications.

Fornecedor: Promega

Descrição: The Access RT-PCR system is designed for the reverse transcription (RT) and PCR amplification of a specific target RNA from total RNA or mRNA. The system uses AMV reverse transcriptase (AMV RT) from Avian Myeloblastosis virus for first-strand DNA synthesis and thermostable Tfl DNA polymerase from <i>Thermus flavus</i> for second-strand cDNA synthesis and DNA amplification. Included is an optimised single-buffer system that permits extremely sensitive detection of RNA transcripts without a requirement for buffer additions between the reverse transcription and PCR amplification steps. This simplifies the procedure and reduces the potential for contaminating the samples.

Fornecedor: Promega

Descrição: GoScript™ Reverse Transcriptase system utilises the M-MLV reverse transcriptase enzyme for reliable cDNA synthesis of a full range of rare and abundant transcripts over a wide range of RNA input amounts (5 µg to 1 pg). The components of the system can effectively reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA. Optimised for use in qPCR, including with GoTaq® Real-Time PCR Systems, it can handle the most challenging templates and is resistant to PCR inhibitors.

Fornecedor: Promega

Descrição: Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5 kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71 kDa. The RNase H activity of M-MLV RT is weaker than that of the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Código de Artigo: (PROMM5313)

Fornecedor: Promega

Descrição: Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5 kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71 kDa. The RNase H activity of M-MLV RT is weaker than that of the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase.

UOM: 1 * 2 mL

Promoção

Fornecedor: Promega

Descrição: The AccessQuick™ RT-PCR system is an easy and convenient master mix for one-tube RT-PCR. It increases the convenience of performing RT-PCR by combining the following components in a single tube, such as Tfl DNA polymerase, dNTPs, magnesium sulphate and reaction buffer. The AMV RT enzyme is provided in a separate tube to allow important no-RT control reactions. Master mix is simply added to RNA templates in reaction vials, followed by the AMV RT, primers and water. It is intended for routine RT-PCR applications that have been previously optimised and do not require extreme conditions.

Fornecedor: Promega

Descrição: Avian Myeloblastosis Virus Reverse Transcriptase (AMV-RT) catalyses DNA polymerisation using template DNA, RNA or RNA:DNA hybrids. It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg²⁺ or Mn²⁺. The enzyme possesses an intrinsic RNase H activity. Both non ionic detergents and sulfhydryl compounds stabilise the enzyme activity <i>in vitro</i>.