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Access RT-PCR system

Fornecedor: Promega

A1250 A1260 A1280
PROMA1250EA 903 EUR
PROMA1250 PROMA12600020 PROMA12800500
Access RT-PCR system
Reagentes de ácidos nucleicos RT-PCR and cDNA Reagents

The Access RT-PCR system is designed for the reverse transcription (RT) and PCR amplification of a specific target RNA from total RNA or mRNA. The system uses AMV reverse transcriptase (AMV RT) from Avian Myeloblastosis virus for first-strand DNA synthesis and thermostable Tfl DNA polymerase from Thermus flavus for second-strand cDNA synthesis and DNA amplification. Included is an optimised single-buffer system that permits extremely sensitive detection of RNA transcripts without a requirement for buffer additions between the reverse transcription and PCR amplification steps. This simplifies the procedure and reduces the potential for contaminating the samples.


  • One tube, two enzyme system provides sensitive, quick and reproducible analysis of even rare RNAs
  • Less template - detect message from as little as 1 pg of total RNA or mRNA
  • Separate tubes of each component provides maximum control for every step of the reaction
  • AMV/Tfl 5X reaction buffer results in optimal enzyme activity without buffer additions between the reverse transcription and DNA amplification steps
  • System tested for performance, it ensures reliable, high-performance results


The improved performance of AMV reverse transcriptase at elevated temperatures in the AMV/Tfl 5X reaction buffer minimises problems encountered with secondary structures in RNA.


Fornecimento: Each kit contains upstream control primer, downstream control primer, positive control RNA with carrier, Access RT-PCR Core Reagents (100 reactions; A1250 minus Controls), AMV reverse transcriptase, AMV/Tfl 5X reaction buffer, dNTP mix, MgSO₄, Tfl DNA polymerase and nuclease free water.

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