RT-PCR system, AccessQuick™
Fornecedor: Promega
The AccessQuick™ RT-PCR system is an easy and convenient master mix for one-tube RT-PCR. It increases the convenience of performing RT-PCR by combining the following components in a single tube, such as Tfl DNA polymerase, dNTPs, magnesium sulphate and reaction buffer. The AMV RT enzyme is provided in a separate tube to allow important no-RT control reactions. Master mix is simply added to RNA templates in reaction vials, followed by the AMV RT, primers and water. It is intended for routine RT-PCR applications that have been previously optimised and do not require extreme conditions.
- Less template: amplify zeptomole (10⁻²¹ mol) levels of RNA
- No buffer additions required between the reverse transcription and DNA amplification steps
- Stability: system components are stable over many freeze-thaw cycles
Fornecimento: The complete system includes AccessQuick™ master mix (2X), AMV reverse transcriptase enzyme and nuclease free water.
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