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Reverse transcription system, ImProm-II™

Fornecedor: Promega

A3800 A3801 A3802 A3803
PROMA3800EA 960 EUR
PROMA3800 PROMA3801 PROMA3802 PROMA3803
Reverse transcription system, ImProm-II™
Reagentes de ácidos nucleicos RT-PCR and cDNA Reagents

The ImProm-II™ reverse transcription system produces efficient, robust synthesis of first-strand cDNA in preparation for PCR amplification. The components can be used to reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA. The optimised reaction buffer and ImProm-II™ Reverse Transcriptase provided in the system together enable robust, full-length cDNA synthesis for the reproducible analysis of rare or long messages. The cDNA synthesis conditions were formulated for standalone applications or for easy transition to gene-specific target amplification. An aliquot of the reverse transcription reaction (1 to 20 μl) can be amplified directly using Taq DNA polymerase in coupled or uncoupled PCR.


  • Amenable to full-length RT-PCR: reverse transcribe long RNA templates up to 8,9 Kb
  • Microarray compatible: may be used to incorporate regular, Cy®3-modified, Cy®5-modified and amino-allyl-modified nucleotides
  • Easy to use: system provides all reagents necessary for efficient reverse transcription
  • Scalable and flexible: 1 to 20 μl of the initial reverse transcription reaction may be used in subsequent PCR, and the optimised buffer allows coupled RT-PCR
  • Versatile: Can be used with thermostable DNA polymerase of choice


Fornecimento: The complete system includes ImProm-II™ Reverse Transcriptase enzyme, ImProm-II™ reaction buffer (5X), MgCl₂, dNTP mix, oligo(dT)15 primer, random primers, upstream control primer, downstream control primer, nuclease-free water, 1,2 kb Kanamycin positive control RNA and recombinant RNasin® ribonuclease inhibitor.

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