Reverse transcription system
Fornecedor: Promega
The Reverse Transcription system provides reagents to efficiently reverse transcribe RNA into cDNA. The cDNA prepared from each reaction using this system may be used directly in multiple PCR amplifications using Taq DNA polymerase. The AMV Reverse Transcriptase synthesises single-stranded cDNA from total or poly(A)+ RNA. Both Oligo(dT)15 and random primers are included, allowing cDNA synthesis from virtually any RNA source. The system contains sufficient reagents for 100 cDNA synthesis reactions, processing 1 μg of RNA per reaction. Each cDNA synthesis reaction may be divided and used in up to 20 separate PCR amplifications.
- Speed: Efficiently reverse transcribe poly(A)+ mRNA or total RNA in 15 minutes
- Positive controls: A polyadenylated RNA transcript is provided to help troubleshoot RT-PCR parameters
Fornecimento: The complete system includes AMV Reverse Transcriptase (HC) enzyme, reverse transcription 10X buffer, MgCl₂, dNTP mix, oligo(dT)15 primer, random primers, nuclease-free water, 1,2 kb Kanamycin positive control RN Aand recombinant RNasin® ribonuclease inhibitor.
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