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AMV reverse transcriptase

Fornecedor: Promega

M5101 M5108 M9004
PROMM51010300EA 174 EUR
PROMM51010300 PROMM51081000 PROMM90040600
AMV reverse transcriptase
Reagentes de ácidos nucleicos RT-PCR and cDNA Reagents

Avian Myeloblastosis Virus Reverse Transcriptase (AMV-RT) catalyses DNA polymerisation using template DNA, RNA or RNA:DNA hybrids. It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg2+ or Mn2+. The enzyme possesses an intrinsic RNase H activity. Both non ionic detergents and sulfhydryl compounds stabilise the enzyme activity in vitro.


  • Efficient reverse transcription of templates with high secondary structure
  • Stable at higher reaction temperatures (37 to 58 °C)


Applications: First-strand synthesis of cDNA from RNA molecules; sequencing of RNA transcripts.


Fornecimento: Kit contains AMV Reverse Transcriptase and AMV RT 5X Reaction Buffer. 5X Reaction Buffer contains 250 mM Tris-HCl (pH 8,3 at 25 °C), 250 mM KCl, 50 mM MgCl2, 2,5 mM spermidine, 50 mM DTT.

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