AMV reverse transcriptase
Fornecedor: Promega
Avian Myeloblastosis Virus Reverse Transcriptase (AMV-RT) catalyses DNA polymerisation using template DNA, RNA or RNA:DNA hybrids. It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg2+ or Mn2+. The enzyme possesses an intrinsic RNase H activity. Both non ionic detergents and sulfhydryl compounds stabilise the enzyme activity in vitro.
- Efficient reverse transcription of templates with high secondary structure
- Stable at higher reaction temperatures (37 to 58 °C)
Applications: First-strand synthesis of cDNA from RNA molecules; sequencing of RNA transcripts.
Fornecimento: Kit contains AMV Reverse Transcriptase and AMV RT 5X Reaction Buffer. 5X Reaction Buffer contains 250 mM Tris-HCl (pH 8,3 at 25 °C), 250 mM KCl, 50 mM MgCl2, 2,5 mM spermidine, 50 mM DTT.
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