Reverse transcriptase, GoScript™
Fornecedor: Promega
GoScript™ Reverse Transcriptase system utilises the M-MLV reverse transcriptase enzyme for reliable cDNA synthesis of a full range of rare and abundant transcripts over a wide range of RNA input amounts (5 µg to 1 pg). The components of the system can effectively reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA. Optimised for use in qPCR, including with GoTaq® Real-Time PCR Systems, it can handle the most challenging templates and is resistant to PCR inhibitors.
- Sensitive: Confident transcription of both high-copy and low-copy messages
- Resilient: Synthesises cDNA in the presence of strong PCR inhibitors
- Processive: Transcribes short and long transcripts
- Consistent: Processes through secondary structure
- Flexible: Available as a complete system or as a stand-alone enzyme
Fornecimento: The complete system includes GoScript™ Reverse Transcriptase enzyme, 5X reaction buffer, MgCl₂, PCR nucleotide mix, oligo(dT)15 primer, random primers, nuclease-free water and recombinant RNasin® ribonuclease inhibitor.
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