Procurou por: Vectors,+Plasmids+and+Libraries

Fornecedor: Cytiva

Descrição: The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.

Fornecedor: Promega

Descrição: The pUC/M13 primers are used to sequence inserts cloned into the M13 vectors and pUC plasmids developed by Messing. These primers also can be used for sequencing other<i> lacZ</i>-containing plasmids such as the pGEM®-Z and pGEM®-Zf Vectors. The primers are purified by gel electrophoresis or HPLC.

Fornecedor: Thermo Fisher Scientific

Descrição: TurboFect™ reagent an easy to use, non-immunogenic transfection reagent for delivery of DNA plasmids and expression vectors into eukaryotic cells.

Fornecedor: Promega

Descrição: S30 T7 high yield protein expression system can be used to express up to 500 μg/ml of protein in 1 hour from plasmid vectors containing a T7 promoter and a ribosome binding site.

Código de Artigo: (PIER34060)

Fornecedor: Thermo Fisher Scientific

Descrição: Thermo Scientific Pierce IPTG is isopropyl-β-D-thiogalactopyranoside for use with beta-galactosidase substrates such as X-Gal to detect expression of vectors, plasmids, or DNA fragments that contain the reporter gene.

UOM: 1 * 1 g

Promoção

Código de Artigo: (PRSI32-166)

Fornecedor: ProSci Inc.

Descrição: Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.

UOM: 1 * 100 µG

Promoção

Fornecedor: Promega

Descrição: The LigaFast™ rapid DNA ligation system is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just five minutes (blunt-ended inserts in as little as 15 minutes).

Fornecedor: SGI - DNA Synthetic Genomics

Descrição: Gibson Assembly® HiFi HC 1-Step Kits and Master Mixes allow restriction digest-free, cloning of one or more DNA fragments into virtually any location of any plasmid vector in a single round of cloning.

Código de Artigo: (PROML11300001)

Fornecedor: Promega

Descrição: The <i>E. coli </i>T7 S30 Extract System for Circular DNA simplifies the transcription/translation of DNA sequences cloned in plasmid or λ vectors containing a T7 promoter by providing an extract that contains T7 RNA polymerase for transcription and all components needed for translation. The investigator only supplies cloned DNA containing a T7 promoter and a ribosome binding site.

UOM: 1 * 30 Tests

Promoção

Código de Artigo: (PROML10200450)

Fornecedor: Promega

Descrição: The <i>E. coli</i> S30 Extract System for Circular DNA simplifies the transcription/translation of DNA sequences cloned in plasmid or λ vectors, providing a powerful tool for identifying and characterising polypeptides. The investigator needs only to supply the cloned DNA containing the appropriate prokaryotic promoter and ribosome binding sites.

UOM: 1 * 450 µl

Promoção

Fornecedor: Corning

Descrição: AxyPrep™ Mag Plasmid Purification Kit utilises a magnetic bead technology for high throughput purification of plasmid DNA from <i>E.coli</i> cells. The AxyPrep™ Mag kit can also be used with fosmid and BAC vector-based constructs ranging from 5 to 150 Kb. The system consists of steps of pelleting cells, resuspension, lysis, neutralisation, DNA binding, ethanol washing and plasmid elution. The protocol is automation-compatible.

Certificados

Fornecedor: MP Biomedicals

Descrição: Storage: Store at -20 °C. Store Desiccated. Store Under Nitrogen. Protect from light.
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, commonly known as X−Gal, is a histochemical substrate for β−galactosidase.
5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. It is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. X−Gal is cleaved by β−galactosidase to yield an insoluble blue precipitate. X−Gal is particularly useful in molecular biology applications to detect the activity of β−galactosidase which is frequently used as a reporter gene. In cloning, X−Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using α-complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β-galactosidase.

Código de Artigo: (ORIGPS100090)

Fornecedor: OriGene

Descrição: AAV Gene Expression Vectors with C-terminal tGFP tag.

UOM: 1 * 10 µG

Promoção

Código de Artigo: (ORIGTR30023)

Fornecedor: OriGene

Descrição: pGFP-C-shLenti vector is a third generation Lentivector which requires the viral components carried in other vectors to produce viral particles. There are three major functional elements within the 5LTR and 3LTR: a shRNA expression cassette driven by an U6 promoter, a puromycin resistant gene driven by a SV40 promoter and a tGFP gene driven by a CMV promoter. All of them can be packaged to viral particles and transduced to many cell lines. The bacterial selection marker for the vector is Chloramphenicol.

UOM: 1 * 5 µG

Novo produto Promoção

Código de Artigo: (13-6945-00)

Fornecedor: VWR Chemicals

Descrição: Isolate up to 40 to 70 µg of high quality plasmid DNA from up to 15 ml bacterial cultures in 30 minutes or less. Simplified with VWR® peqGold Mini Column II technology into three quick steps: Bind, wash and elute.

UOM: 1 * 5 Tests

MSDS Certificados Promoção

Código de Artigo: (13-6943-00)

Fornecedor: VWR Chemicals

Descrição: Plasmid Miniprep Kit I for the easy, quick and efficient extraction of highly pure plasmid DNA. Isolate up to 30 µg of high quality plasmid DNA from 1 to 5 ml bacterial cultures in 30 minutes or less. Plasmid DNA obtained with peqGOLD Plasmid Miniprep kits can directly be used for a wide range of downstream applications.

UOM: 1 * 5 Tests

MSDS Certificados Promoção