Rapid DNA ligation system, LigaFast™
Fornecedor: Promega
The LigaFast™ rapid DNA ligation system is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just five minutes (blunt-ended inserts in as little as 15 minutes).
- DNA inserts with blunt ends or 5´ or 3´ overhangs can be used for ligation
- No purification of ligated DNA needed prior to heat-shock transformation in E. coli
- Ligations performed using the LigaFast™ system are comparable to standard overnight ligations
Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X rapid ligation buffer. The LigaFast™ system is designed to eliminate any further purification prior to transformation of ligated DNA. The specially formulated 2X rapid ligation buffer requires no additional ATP or Mg²⁺ addition prior to use.
The LigaFast™ System is blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications.
Storage buffer: 10 mM Tris-HCl (pH 7,4 at 25 °C), 50 mM KCl, 1 mM DTT, 0,1 mM EDTA and 50% glycerol.
Fornecimento: Kit includes 2X rapid ligation buffer and T4 DNA ligase.
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