Procurou por: Cloning Kits and Ligation

Fornecedor: Thermo Fisher Scientific

Descrição: The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host <i>E. coli</i> cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

Código de Artigo: (EL0016)

Fornecedor: Thermo Fisher Scientific

Descrição: This T4 DNA ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.

UOM: 1 * 1 KIT

Certificados Promoção

Fornecedor: SGI - DNA Synthetic Genomics

Descrição: The Gibson Assembly® Ultra reaction occurs in two steps using the addition of two master mixes in two sequential steps. In the first step, the GA Ultra Master Mix A (2X) creates single-strand DNA 5’ overhangs by chewing back DNA from the 3’ end. This reaction is cooled under conditions that allow for annealing of complementary overlap regions. Next, the Gibson Assembly® Ultra Master Mix B (2X) is added. During this step, nucleotides are incorporated into the construct to fill in the gaps in the annealed DNA fragments and nicks are sealed to create a contiguous DNA construct.

MSDS

Fornecedor: SGI - DNA Synthetic Genomics

Descrição: The Gibson Assembly® HiFi 1 Step Kit provides a simple and effective method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method allows researchers to achieve complex assembly of large DNA constructs, with multiple inserts, in 1 hour.

MSDS

Fornecedor: SGI - DNA Synthetic Genomics

Descrição: Gibson Assembly® HiFi HC 1-Step Kits and Master Mixes allow restriction digest-free, cloning of one or more DNA fragments into virtually any location of any plasmid vector in a single round of cloning.

Fornecedor: Thermo Fisher Scientific

Descrição: The aLICator™ LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in <i>E. coli</i>. The plate bacterial expression vectors are designed for high levels of target protein expression in concert with minimal basal (uninduced) expression, which permits expression of proteins that are toxic to <i>E. coli</i> cells.

Fornecedor: SGI - DNA Synthetic Genomics

Descrição: The Gibson Assembly® Hi-Fi 1-Step Master Mix (2X) contains a proprietary mixture of enzymes and reagents optimised to facilitate one-step assembly of double standed DNA fragments. This master mix includes a proof-reading polymerase that mediates junction repair resulting assembled constructs with low rates of junction errors and high sequence fidelity.

MSDS

Fornecedor: Merck Millipore (Novagen)

Descrição: Clonables™ 2X ligation premix is a single solution containing optimised concentrations of T4 DNA ligase, buffer, stabiliser, and cofactors needed for efficient ligation of any type of compatible DNA ends.

Fornecedor: SGI - DNA Synthetic Genomics

Descrição: The Gibson Assembly® Ultra Kit provides a robust and efficient method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method is designed for complex assembly of 2 to 15 large DNA constructs using only small amounts (nanograms) of DNA.

MSDS

Fornecedor: Merck Millipore (Novagen)

Descrição: Novagen® T7Select® Phage Display System takes advantage of the properties of bacteriophage T7. This system is easy to use and has the capacity to display peptides up to about 50 amino acids in size in high copy number (415 per phage), and peptides or proteins up to about 1200 amino acids in low copy number (0,1 to 1 per phage) or mid-copy number (5 to 15 per phage).

Código de Artigo: (27-0361-01)

Fornecedor: Cytiva

Descrição: T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction.

UOM: 1 * 50 Tests

Promoção

Código de Artigo: (PROMM10510500)

Fornecedor: Promega

Descrição: T4 RNA ligase catalyses the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from recombinant <i>E. coli</i> CA4 (RNase I-deficient), has an apparent molecular weight of 43,5 kDa. It also catalyses the addition of [5'-³²P] nucleoside 3',5'-bis (phosphate) onto single-stranded RNA.

UOM: 1 * 500 UE

Promoção

Fornecedor: Promega

Descrição: The LigaFast™ rapid DNA ligation system is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just five minutes (blunt-ended inserts in as little as 15 minutes).

Código de Artigo: (PROMM1794)

Fornecedor: Promega

Descrição: T4 DNA Ligase catalyses the joining of two cohesive- or blunt-ended strands of DNA between the 5'-phosphate and the 3'-hydroxyl groups of adjacent nucleotides. T4 DNA Ligase is provided with 10X reaction buffer: 300 mM Tris-HCl (pH 7,8 at 25 °C), 100 mM MgCl₂, 100 mM DTT and 10 mM ATP.

UOM: 1 * 500 UE

Promoção

Fornecedor: Promega

Descrição: T4 DNA Ligase catalyses the joining of two cohesive- or blunt-ended strands of DNA between the 5'-phosphate and the 3'-hydroxyl groups of adjacent nucleotides. T4 DNA Ligase is provided with 10X reaction buffer: 300 mM Tris-HCl (pH 7,8 at 25 °C), 100 mM MgCl₂, 100 mM DTT and 10 mM ATP.

Código de Artigo: (PROMP97510500)

Fornecedor: Promega

Descrição: Useful host for transformation of pGEM Vectors and production of ssDNA from M18 or phagemid vectors. JM109 is recA- and lacks the E. coli K restriction system. Can be used for blue/white screening.

UOM: 1 * 500 µl

Promoção