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T4 DNA ligase (from Escherichia coli)

Fornecedor: Promega

M1801 M1804
PROMM18010100EA 78.8 EUR
PROMM18010100 PROMM18040500
T4 DNA ligase (from Escherichia coli)
Reagentes de ácidos nucleicos Cloning Kits and Ligation

T4 DNA Ligase catalyses the joining of two cohesive- or blunt-ended strands of DNA between the 5'-phosphate and the 3'-hydroxyl groups of adjacent nucleotides. T4 DNA Ligase is provided with 10X reaction buffer: 300 mM Tris-HCl (pH 7,8 at 25 °C), 100 mM MgCl₂, 100 mM DTT and 10 mM ATP.


  • Use with 5', 3' or blunt-ended dsDNA
  • Qualified for blue/white cloning
  • Providing a higher level of quality control for enzymes used in cloning applications
  • It can catalyse the joining of RNA to either a DNA or RNA strand in a duplex molecule
  • Will not join single-stranded nucleic acids


Storage buffer: 10 mM Tris-HCl (pH 7,4 at 25 °C), 50 mM KCl, 1 mM DTT, 0,1 mM EDTA and 50% glycerol.


Unit definition: 0,01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyse the ligation of greater than 95% of 1 μg of λ/HindIII fragments at 16 °C in 20 minutes.

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