Procurou por: Aluminum+oxide-Silicon+oxide

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β-Nicotinamida adenina dinucleotídeo (NAD, forma oxidada) ≥98%, pó branco reagente para cultura de célula

Fornecedor: MP Biomedicals

Descrição: Storage: -20°C, desiccate
This is an ultrapure NAD, chromatographically purified to remove trace inhibitors.
β-NAD, a pyridine nucleotide and biologically active form of nicotinic acid, is a coenzyme necessary for the catalytic reaction of certain enzymes. It occurs in living cells primarily in the oxidized state. Serves as a coenzyme of the dehydrogenases, especially in the dehydrogenation of primary and secondary alcohols. NAD usually acts as a hydrogen acceptor, forming NADH which then serves as a hydrogen donor in the respiratory chain.
Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NAD as a catalyst intermediate and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. The reduced form, β-NADH, is fluorescent whereas β-NAD is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10-7 M.
Electron acceptor. β-NAD is a carrier for hydride ion, forming b-NADH. Hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as malic dehydrogenase and lactic dehydrogenase. Such enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to b-NAD to form the reduced product, b-NADH. Unlike b-NAD which has no absorbance at 340 nm, b-NADH absorbs at 340 nm (EmM = 6.22). The increase in absorbance at 340 nm with the formation of b-NADH is the basis for measurement of activity of many enzymes.

Sigmacote® siliconizing reagent

Fornecedor: SIGMA ALDRICH MICROSCOPY

Descrição: Siliconizing reagent for glass and other surfaces. Sigmacote® is a solution of a chlorinated organopolysiloxane in heptane that readily forms a covalent, microscopically thin film on glass.

Nitric oxide reagents and kits

Fornecedor: Biotium

Descrição: Nitric oxide donors, nitric oxide synthase inhibitors and nitric oxide detection reagents and kits for nitric oxide (NO) studies.

Polyethylene glycol/oxide standards

Fornecedor: VARIAN

Descrição: These hydrophilic polymers are suitable for both aqueous SEC and organic GPC using the majority of polar organic solvents. The two types are chemically similar that can be used together across a wider molecular weight range with aqueous and organic polymers.