Procurou por: Merck Millipore (Novagen)

Fornecedor: Merck Millipore (Novagen)

Descrição: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Código de Artigo: (70607-3)

Fornecedor: Merck Millipore (Novagen)

Descrição: Loading Buffer, 4X SDS sample buffer

UOM: 1 * 2 mL

Promoção

Fornecedor: Merck Millipore (Novagen)

Descrição: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Código de Artigo: (69660-3)

Fornecedor: Merck Millipore (Novagen)

Descrição: The pET-14b vector carries an N-terminal His·Tag® sequence followed by a thrombin cleavage site and three cloning sites.

UOM: 1 * 10 µG

Promoção

Código de Artigo: (69203-3)

Fornecedor: Merck Millipore (Novagen)

Descrição: Streptavidin agarose consists of cross-linked agarose covalently coupled with pure streptavidin under controlled conditions. The stable linkage to the resin minimizes leaching of the streptavidin while maintaining full binding activity. The matrix is suitable for use in column and batch formats for any application that requires high biotin binding capacity and low non-specific binding. The resin has no detectable protease, DNase, or RNase contamination.

UOM: 1 * 5 mL

Promoção

Código de Artigo: (71546-3)

Fornecedor: Merck Millipore (Novagen)

Descrição: The Novagen® BacMagic™-1 transfection kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

UOM: 1 * 1 KIT

Promoção

Fornecedor: Merck Millipore (Novagen)

Descrição: NovaBlue competent cells are designed for convenient and reliable plasmid transformation. NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids), and <i>recA</i> and <i>endA</i> mutations, which result in high yields of excellent-quality plasmid DNA. Standard NovaBlue and convenient NovaBlue Singles™ are ideal for routine cloning, while NovaBlue GigaSingles™ are most suitable for applications requiring higher transformation efficiencies.

Código de Artigo: (71004-3)

Fornecedor: Merck Millipore (Novagen)

Descrição: A mistura dNTP mix de 10 mM é uma preparação pronta a usar de dATP, dCTP, dGTP e dTTP (sais monossódicos) ultrapuros, com uma concentração de 10 mm cada, em água desionizada esterilizada com um pH de 7,0. Indicada para qualquer aplicação que necessite de desoxinucleótidos puros, tais como a PCR, a síntese de ADNc, e reacções de preenchimento.

UOM: 1 * 0,2 mL

Promoção

Fornecedor: Merck Millipore (Novagen)

Descrição: Pellet Paint® Co-Precipitant is a visible dye-labelled carrier formulated specifically for use in alcohol precipitation of nucleic acids. The 2-minute precipitation does not require low-temperature incubations or prolonged centrifugation. Both RNA and DNA are efficiently precipitated from even the most dilute solutions (2 ng/ml) and the pellet is easily located by its vivid pink colour. The pellet can be easily followed during washing steps and this prevents losses during handling and ensures thorough pellet re-suspension.

Fornecedor: Merck Millipore (Novagen)

Descrição: O reagente para extracção de proteínas BugBuster® Protein foi formulado de modo a fraccionar suavemente a parede das células de <i>E. coli</i>, libertando proteína solúvel. Constitui uma alternativa simples, rápida e económica a métodos mecânicos como, p. ex., French Press ou sonicação, para libertar proteína alvo expressa, na preparação para a purificação ou outras aplicações. A formulação utiliza uma mistura de detergentes capaz de perfurar a parede das células sem desnaturar a proteína solúvel.

Código de Artigo: (71285-3)

Fornecedor: Merck Millipore (Novagen)

Descrição: BCA Protein Assay is a simple and reliable protein quantification method. It can be used to determine protein concentration in the range of 20-2000 µg/ml in either a standard assay or microassay configuration.

UOM: 1 * 1 KIT

Promoção

Código de Artigo: (70976-3)

Fornecedor: Merck Millipore (Novagen)

Descrição: The SpinPrep™ PCR Clean Up Kit is designed for the rapid purification of DNA amplified by PCR. The 10-minute procedure involves addition of a binding buffer followed by adsorption of the DNA to a silica membrane in a spin column format. Following a wash step, the DNA is eluted in low salt buffer. This kit removes DNA polymerises, dNTPs, salts, and >99% of primers so that they do not interfere with downstream applications such as cloning, sequencing, or labelling. PCR products from 100 to >12000 bp can be cleaned up, with recoveries of amplified DNA in the range of 60 to 90% under standard conditions.

UOM: 1 * 100 Tests

Promoção

Fornecedor: Merck Millipore (Novagen)

Descrição: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Código de Artigo: (69022-3)

Fornecedor: Merck Millipore (Novagen)

Descrição: The thrombin cleavage capture kit is designed for cleavage of fusion proteins followed by convenient and quantitative removal of thrombin protease. The method is based on the use of biotinylated thrombin for digestion and its subsequent removal with streptavidin agarose.

UOM: 1 * 1 KIT

Promoção

Código de Artigo: (71366-4)

Fornecedor: Merck Millipore (Novagen)

Descrição: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

UOM: 1 * 1 unid.

Promoção

Código de Artigo: (71610-3)

Fornecedor: Merck Millipore (Novagen)

Descrição: D-Desthiobiotin provides a gentle elution of StrepTag® II proteins from the biotin-binding site of StrepTactin® resins. D-Desthiobiotin is offered as a lyophilised powder for preparation of elution buffer.

UOM: 1 * 1 g

Promoção