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Procurou por: Reagentes+de+Purificação+de+Proteínas


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Código de Artigo: (SOLUK-7413-25)
Fornecedor: TriLink BioTechnologies
Descrição: <p><span style="color:black">TriLink’s CleanCap® AG (3′ OMe) CleanScript™ IVT kit provides essential components for mRNA synthesis using co-transcriptional capping by in vitro transcription (IVT). The kit components are sufficient to run 25 of 100 µl reactions following our recommended IVT protocol with co-transcriptional capping. It includes a cap analog, modified and unmodified nucleotides, IVT enzymes including CleanScribe RNA polymerase, 10X CleanScript IVT reaction buffer, and a linearized FLuc DNA control template.</span></p>
UOM: 1 * 100 µl

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Fornecedor: G-Biosciences
Descrição: Concanavalin A (Con A) agarose consists of Con A coupled to 6% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from <i>Canavalia ensiformis</i> (jack bean). It is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. It has also be used in other application areas including purification of enzyme-antibody conjugates, purification of IgM and separation of membrane vesicles. As stated above, it is a metalloprotein and to maintain its binding characteristics the presence of both Mn²⁺ and Ca²⁺ is essential. Each subunit of Con A utilises one calcium and one manganese ion and these cations can be removed under acidic conditions abolishing the carbohydrate-binding activity.

Código de Artigo: (220947S)
Fornecedor: VWR Chemicals
Descrição: Biureto solução reagente para análise de proteína (total) in soro ou plasma
UOM: 1 * 5 L

MSDS Certificados


Fornecedor: Merck Millipore (Novagen)
Descrição: GST Bind™ Resin utilizes an 14-atom spacer arm to covalently attach reduced glutathione via a sulfide linkage. The high degree of substitution of glutathione ensures a high binding capacity with yields of glutathione S-transferase (GST) fusion proteins of 5 to 8 mg/ml settled resin. The resin can be re-used up to 6 times without loss of capacity.

Código de Artigo: (PIER20372)
Fornecedor: Thermo Fisher Scientific
Descrição: Immobilised D-Galactose agarose enables purification of lectins, galactosidases and other galactose-binding molecules. The immobilised D-galactose is a beaded agarose affinity resin that enables purification of agglutinins, lectins, toxins, and other galactose-binding proteins, N-acetylgalactosamine-binding proteins or carbohydrate-binding proteins.
UOM: 1 * 5 mL


Fornecedor: Cytiva
Descrição: MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.
Fornecedor: Thermo Fisher Scientific
Descrição: The HisPur™ Ni-NTA Spin Purification Kit provides a fast, easy to use format for routine affinity purification of His-tagged fusion proteins and contains 0,2-ml HisPur™ Ni-NTA spin columns, as well as buffers required to purify polyhistidine-tagged proteins.

Código de Artigo: (17-0906-01)
Fornecedor: Cytiva
Descrição: NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.
UOM: 1 * 25 mL

MSDS Certificados


Fornecedor: Cytiva
Descrição: Ni Sepharose™ excel is an IMAC medium pre-charged with nickel ions very strongly bound to a chelating ligand. Samples that usually cause stripping of metal ions can, therefore, be loaded to the medium. Ni Sepharose™ excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.

Fornecedor: G-Biosciences
Descrição: The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.

Código de Artigo: (TCIAB5702-500ML)
Fornecedor: TCI
Descrição: Bradford Assay Solution (Ready-to-use)
UOM: 1 * 500 mL


Fornecedor: Merck Millipore (Novagen)
Descrição: O reagente para extracção de proteínas BugBuster® Protein foi formulado de modo a fraccionar suavemente a parede das células de <i>E. coli</i>, libertando proteína solúvel. Constitui uma alternativa simples, rápida e económica a métodos mecânicos como, p. ex., French Press ou sonicação, para libertar proteína alvo expressa, na preparação para a purificação ou outras aplicações. A formulação utiliza uma mistura de detergentes capaz de perfurar a parede das células sem desnaturar a proteína solúvel.

Código de Artigo: (17-0480-01)
Fornecedor: Cytiva
Descrição: Epoxy-activated Sepharose™ 6B is a pre-activated medium for immobilisation of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose™ 6B via a 12-atom hydrophilic spacer arm. Medium is formed by reacting Sepharose™ 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. It can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.
UOM: 1 * 15 g


Código de Artigo: (69203-3)
Fornecedor: Merck Millipore (Novagen)
Descrição: Streptavidin agarose consists of cross-linked agarose covalently coupled with pure streptavidin under controlled conditions. The stable linkage to the resin minimizes leaching of the streptavidin while maintaining full binding activity. The matrix is suitable for use in column and batch formats for any application that requires high biotin binding capacity and low non-specific binding. The resin has no detectable protease, DNase, or RNase contamination.
UOM: 1 * 5 mL


Fornecedor: Cytiva
Descrição: rProtein A Sepharose™ 4 Fast Flow is a well established antibody affinity medium is designed for the purification of monoclonal and polyclonal antibodies based on Sepharose™ Fast Flow platform. The recombinant protein A is produced in <i>E.coli</i> and engineered for an oriented coupling to giving a matrix with enhanced binding capacity. The epoxy-based coupling ensures low ligand leakage. The specificity of the recombinant protein A for the Fc region of IgG is similar to native protein A and provides excellent purification in one step. The high capacity, low ligand leakage and a well established base matrix makes rProtein A Sepharose™ Fast Flow ideal for purification of monoclonal antibodies at both laboratory and process scale.

Fornecedor: G-Biosciences
Descrição: Recombinant streptavidin from S<i>treptomyces avidinii</i>, produced in <i>E.coli.</i>

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O stock para este item é limitado. Por favor, certifique-se de que efetuou o seu login para visualizar o stock disponível. Se a call ainda é exibida e precisar de ajuda, por favor, ligue para 213 600 770
O stock para este item é limitado. Por favor, certifique-se de que efetuou o seu login para visualizar o stock disponível. Se a call está visível e precisar de ajuda, por favor, ligue para 213 600 770
Este produto é sujeito a regulamentação especifica.
Em caso de encomenda, será contactado a solicitar documentação complementar necessária e/ou obrigatória (licença, autorização ou declaração de uso final) para a continuidade do pedido. Agradecemos a vossa compreensão
Este produto é sujeito a regulamentação especifica.
Em caso de encomenda, será contactado a solicitar documentação complementar necessária e/ou obrigatória (licença, autorização ou declaração de uso final) para a continuidade do pedido. Agradecemos a vossa compreensão.
Este produto está bloqueado. Para obter mais informações, contacte a VWR através do número 213 600 770.
O produto pretendido já não se encontra disponível. O produto indicado é um substituto.
Este produto encontra-se em rutura de stock. Poderá encontrar alternativas pesquisando pelo código de artigo indicado acima. Se precisar de ajuda, por favor contacte a VWR através do 213 600 770.
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