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Imagem do Produto-K9012

Master mixes, DreamTaq™ Hot Start PCR master mix (2X)

Fornecedor: Thermo Fisher Scientific
Descrição: Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mix is a ready to use 2X reaction mix that includes DreamTaq™ Hot Start DNA Polymerase, DreamTaq™ buffer, magnesium, and dNTPs. The master mix allows easy reaction setup with fewer pipetting steps, with only primers, template, and water needing to be added. The green master mix includes density reagent and two tracking dyes for convenient direct loading of PCR products on a gel.

Imagem do Produto-F-549L

Phusion® Hot Start II high-fidelity DNA polymerase

Fornecedor: FINNZYMES REAGENTS
Descrição: Phusion® Hot Start II High-Fidelity DNA Polymerase allows the use of a wide range of primers, including those with low melting temperatures. Phusion® Hot Start II High-Fidelity DNA Polymerase is ideal for PCR applications from routine PCR to highly demanding applications, such as cloning and high throughput PCR. It incorporates a unique dsDNA-binding domain making this Pyrococcus-like proofreading DNA polymerase extremely robust, rapid, and accurate.

Imagem do Produto-F-566L

Phusion® green hot start II high-fidelity PCR master mix

Fornecedor: FINNZYMES REAGENTS
Descrição: Phusion® green hot start II high-fidelity PCR master mix is a convenient 2X mix designed to minimise the number of pipetting steps. The master mix contains Phusion Hot Start II DNA Polymerase, nucleotides and optimised reaction buffer including MgCl₂. The buffer also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel.

Imagem do Produto-BE-308

Experimentation kits, DNA ligation

Código de Artigo: (BE-308)
Fornecedor: G-Biosciences
Descrição: Following restriction enzyme digestion of DNA molecules, researchers need to rejoin the ends of the DNA to generate recombinant DNA, a process known as ligation. Ligation of DNA is achieved with the bacterial enzyme T4 DNA ligase, which catalyses the formation of phosphodiester bonds. The DNA Ligation kit teaches students about ligation as they ligate several DNA fragments together to make larger pieces of DNA that are easily identified by agarose electrophoresis.
UOM: 1 * 1 KIT
Imagem do Produto-710-1729

Real-Time PCR kit for detection of Salmonella

Fornecedor: Q BIOANALYTIC
Descrição: OneCup it‘s so easy! The OneCup assay comes along with a minimum of pipetting steps. Everything is included in the mix. You only need to combine 20 μl of the mix with 2 μl of your DNA sample and everything is ready. You have a minimum of hands-on time and avoid mistakes during pipetting. You can run the test on all common block cyclers. Application: Detection of Salmonella in food samples and environmental samples subsequent to a pre-enrichment step according to DIN EN ISO 20837 and 20838. In combination with an appropriate pre-enrichment and DNA preparation the test complies with the official method of the German food and feed law §64. ASU L00.00-98. Test principle: The test is dedicated to detect DNA of Salmonella in a sample using Real-Time PCR in compliance with the ISO method mentioned below. The kit uses the TaqMan® principle. Thus the requirements of the ISO standard, that an amplification product has to be confirmed by a hybridization step, is fulfilled. Each reactions contains an internal amplification control. Therefore, false negative results due to inhibition of the reaction can be excluded. The test included the UNG enzyme to prevent re-amplification of contaminating amplicons.

Imagem do Produto-F-537L

Phusion® Green Hot Start II High-Fidelity DNA Polymerase

Fornecedor: FINNZYMES REAGENTS
Descrição: Phusion® Green Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion® Hot Start II DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® Hot Start II DNA polymerase and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Imagem do Produto-F-122S

Phire® Hot Start II DNA polymerase

Fornecedor: FINNZYMES REAGENTS
Descrição: Phire® Hot Start II DNA Polymerase is faster, extremely robust, and capable of amplifying long DNA fragments with high yields. Phire® Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to <i>Taq</i> DNA polymerase. In addition, the Hot Start technology allows complete reactivation of the enzyme in “zero-time” at standard cycling temperatures. This combination of features makes the polymerase an ideal solution for routine and high throughput PCR applications. Phire® Hot Start II DNA Polymerase delivers superior performance in conventional thermal cyclers as well as in fast instruments, such as the Piko® Thermal Cycler.

Imagem do Produto-F-548L

Phusion® Flash high-fidelity PCR master mix

Fornecedor: FINNZYMES REAGENTS
Descrição: Phusion® Flash High-Fidelity PCR Master Mix is a 2X master mix enabling the use of extremely short PCR protocols without compromising either the fidelity or the yield in the reaction. The master mix utilises Phusion® Flash II DNA Polymerase, a proofreading DNA polymerase modified from Phusion® Hot Start II High-Fidelity DNA Polymerase. The fidelity of the Phusion® Flash II DNA Polymerase is 25-fold greater than <i>Taq</i>.

Imagem do Produto-K1081

DreamTaq™ Green PCR Master Mix (2X)

Fornecedor: Thermo Fisher Scientific
Descrição: DreamTaq Green PCR Master Mix (2X) is a ready to use solution containing DreamTaq™ DNA polymerase, optimised DreamTaq Green buffer, MgCl<sub>2</sub> and dNTPs. The master mix is supplemented with two tracking dyes and a density reagent that allows for direct loading of the PCR product on a gel. The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications, such as DNA sequencing, ligation and restriction digestion. The master mix retains all features of DreamTaq DNA polymerase. It is capable for robust amplification up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

Certificados

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Mag-Bind® Endo-Free plasmid 96 kit

Fornecedor: OMEGA BIO-TEK
Descrição: The Mag-Bind® Endo-Free plasmid 96 kit combines the power of Mag-Bind® technology with the innovative ETR technology to deliver high quality endotoxin-free plasmid DNA in a high throughput format. By adding a quick endotoxin removal step, the Mag-Bind® Endo-Free plasmid 96 kit provides a flexible and fast solution for isolating endotoxin free plasmid on a small economical scale. The flexible system can be easily automated, thereby making it an ideal option for high throughput transfection screening as well as for other standard molecular biology techniques including restriction enzyme digestion.

Imagem do Produto-D3392-02

Mini kit para extracção de ADN de sangue E.Z.N.A.®

Código de Artigo: (D3392-02)
Fornecedor: OMEGA BIO-TEK
Descrição: O E.Z.N.A.® Blood DNA Kit fornece rápido isolamento total de DNA de até 250 µl de sangue total anticoagulado fresco ou congelado. O E.Z.N.A.® Blood DNA Kit também pode ser usado para a preparação de DNA genômico de buffy coat, soro, plasma, medula óssea, linfócitos, plaquetas e fluidos corporais. Este kit permite o processamento simultâneo de amostras únicas ou múltiplas em menos de 30 minutos. Extrações de fenol/clorofórmio e etapas demoradas, como precipitação com isopropanol ou etanol, foram eliminadas. O DNA purificado com o método E.Z.N.A.® Blood DNA está pronto para aplicações como PCR, Southern blotting ou digestão com enzimas de restrição.
UOM: 1 * 200 Tests

MSDS Certificados


Imagem do Produto-28-9034-71

illustra™ GFX™ PCR DNA and gel band purification kit

Fornecedor: Cytiva
Descrição: The illustra GFX PCR DNA and Gel Band Purification kits are for the isolation and concentration of DNA fragments from PCR mixtures, DNA-containing agarose gel bands, enzyme-based DNA modifications, and restriction enzyme digests.

Imagem do Produto-D6922-00

E.Z.N.A.® Plasmid Maxi kit

Fornecedor: OMEGA BIO-TEK
Descrição: The E.Z.N.A.® Plasmid Maxi Kit is the maxi scale system to deliver high-quality plasmid DNA using the spin column format, without costly accessories. The HiBind® DNA Maxi column facilitates the binding, washing, and elution steps, thus enabling multiple samples to be simultaneously processed. Although yields vary according to plasmid copy number, <i>E.coli</i> strain, and conditions of growth, 50 to 200 ml of overnight culture in LB medium typically produces 0,5 to 1 mg of high copy number plasmid DNA. Up to 500 ml of culture may be processed when working with low copy number plasmid. The product is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, transfection of mammalian cells, transcription<i> invitro</i>, and other applications.

MSDS Certificados

Image Unavailable-M7763-01

Mag-Bind® PX blood RNA 96 kit

Fornecedor: OMEGA BIO-TEK
Descrição: The Mag-Bind® PX blood RNA kit allows for the isolation of total RNA from 2,5 ml of whole blood that has been stored in PAXgene® Blood RNA tubes. Stabilised blood samples are first spun down and the nucleic acid pellets are washed and recollected. After washing, the nucleic acid pellet is dissolved into a specially formulated resuspension buffer. At this point the samples can be transferred to 96-well plates for processing in robotic liquid handlers and magnetic processors. High quality intact RNA can be isolated in less than 90 minutes.

Image Unavailable-71007-3

NovaTaq PCR master mix

Código de Artigo: (71007-3)
Fornecedor: Merck Millipore (Novagen)
Descrição: The NovaTaq PCR Master Mix is a ready to use 2X concentrated mixture of NovaTaq DNA polymerase, ultra-pure deoxynucleotides, and reaction buffer without magnesium chloride. The master mix simplifies the assembly of PCR reactions and offers advantages of times savings, consistency, and minimal risk of contamination. Simply add the NovaTaq PCR master mix to an equal volume containing the required amount of magnesium chloride, DNA template, and primers, and the reaction is ready for thermal cycling. The final diluted reaction contains 2,5 units of NovaTaq DNA polymerase per 100 µl. Sufficient components are included for 200 standard 50 µl (or 100×100 µl) amplification reactions.
UOM: 1 * 250 UE
Imagem do Produto-K1682

Maxima™ H minus first strand cDNA synthesis kits

Fornecedor: Thermo Fisher Scientific
Descrição: Maxima™ H Minus First Strand cDNA Synthesis Kit is a complete system for highly efficient synthesis of first strand cDNA. The kit uses the Maxima™ H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima™ H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65 °C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.

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O stock para este item é limitado. Por favor, certifique-se de que efetuou o seu login para visualizar o stock disponível. Se a call ainda é exibida e precisar de ajuda, por favor, ligue para 213 600 770
O stock para este item é limitado. Por favor, certifique-se de que efetuou o seu login para visualizar o stock disponível. Se a call está visível e precisar de ajuda, por favor, ligue para 213 600 770
Este produto é sujeito a regulamentação especifica.
Em caso de encomenda, será contactado a solicitar documentação complementar necessária e/ou obrigatória (licença, autorização ou declaração de uso final) para a continuidade do pedido. Agradecemos a vossa compreensão
Este produto é sujeito a regulamentação especifica.
Em caso de encomenda, será contactado a solicitar documentação complementar necessária e/ou obrigatória (licença, autorização ou declaração de uso final) para a continuidade do pedido. Agradecemos a vossa compreensão.
Este produto está bloqueado. Para obter mais informações, contacte a VWR através do número 213 600 770.
O produto pretendido já não se encontra disponível. O produto indicado é um substituto.
Este produto encontra-se em rutura de stock. Poderá encontrar alternativas pesquisando pelo código de artigo indicado acima. Se precisar de ajuda, por favor contacte a VWR através do 213 600 770.
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