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Procurou por: End-point+PCR+Enzymes+and+Kits


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Código de Artigo: (ENZOENZ4273033)
Fornecedor: ENZO LIFE SCIENCES
Descrição: Terminal labeling is an ideal procedure for the labeling of oligonucleotides with haptens such as biotin or fluorescein. An oligonucleotide can be synthesized using standard, commercially available reagents and labeled after synthesis in a simple and reproducible enzyme reaction.
UOM: 1 * 1 KIT


Código de Artigo: (733-1271)
Fornecedor: Quantabio
Descrição: A dNTP Mix de 10 mM é uma solução de desoxirribonucleósido-trifosfatos de elevada pureza, funcionalmente qualificada para PCR quantitativa em tempo real (qPCR). Está indicada para uso em PCR convencionais de ponto final, qPCR em tempo real, síntese de ADNc da primeira cadeia, bem como noutras aplicações que necessitem do dNTP como substrato. O uso de dNTPs certificados para qPCR é uma medida simples mas importante para garantir um desempenho consistente do ensaio.
UOM: 1 * 1.000 µl


Fornecedor: Quantabio
Descrição: The qScript™ XLT One-Step RT-PCR Kit is a convenient and highly sensitive system for amplification of RNA templates up to 2 kb. cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. This system has been optimised to deliver maximum endpoint RT-PCR efficiency, sensitivity, and specificity.
Fornecedor: Quantabio
Descrição: A síntese do cDNA e a amplificação da PCR são efectuadas no mesmo tubo, num passo único. Estes kits estão optimizados para proporcionar a máxima eficiência e especificidade na RT-PCR, permitindo uma co-amplificação precisa das transcrições com poucas cópias na presença de genes de referência com um número de cópias superior. Em resultado disso, os kits de qRT-PCR qScript™ One-Step apresentam uma sensibilidade superior à de outros reagentes para qPCR disponíveis no mercado. O qScript™ One-Step Master Mix maximiza as actividades das enzimas da transcriptase inversa e da PCR, minimizando simultaneamente a formação do dímero iniciador Especificidade elevada garantida com a AccuStart™ Taq DNA polymerase (polimerase do DNA termoestável), cuja ligação se dá por anticorpos monoclonais para um arranque a quente (Hot-Start) superior. Após o aquecimento a 95 °C, os anticorpos desnaturam-se irreversivelmente, libertando Taq DNA polymerase (DNA polimerase termoestável) não modificada e integralmente activa. Estão disponíveis kits de qRT-PCR qScript™ One-Step para todas as plataformas de instrumentos para PCR em tempo real, incluindo os que requerem normalização com o corante de referência ROX.
Código de Artigo: (ENZOENZ4273031)
Fornecedor: ENZO LIFE SCIENCES
Descrição: Terminal labeling is an ideal procedure for the labeling of oligonucleotides with haptens such as biotin or fluorescein. An oligonucleotide can be synthesized using standard, commercially available reagents and labeled after synthesis in a simple and reproducible enzyme reaction.
UOM: 1 * 1 KIT


Fornecedor: Quantabio
Descrição: AccuStart™ II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart™ II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.
Código de Artigo: (TNB-6611-KIT)
Fornecedor: Tonbo Biosciences
Descrição: The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.
UOM: 1 * 50 Tests


Código de Artigo: (733-2265)
Fornecedor: Quantabio
Descrição: PerfeCTa® PreAmp SuperMix is a 5X concentrated, ready to use reaction cocktail for unbiased, selected enrichment of target sequences from limited amounts of starting material for downstream gene expression profiling or targeted resequencing. It contains all components, except primers and templates. The 5X concentrated SuperMix allows addition of higher template volumes when working with low concentration samples, and/or reduced reaction volumes. Inclusion of an inert light blue tracer dye helps visualise small reaction volumes and ensure accurate pipetting.
UOM: 1 * 40 Assays


Código de Artigo: (BE-314)
Fornecedor: G-Biosciences
Descrição: A major challenge for molecular biologists and genetic engineers is to easily detect and analyze genetic mutations that occur naturally, causing diseases, or during genetic engineering or cloning, whether deliberate or accidental. Students learn about different types of genetic mutations, including substitutions, deletions and point mutations and about various techniques used to detect genetic mutations. The kit contains all the reagents to screen simulated clinical samples for a mutant gene, using both the polymerase chain reaction (PCR) and restriction digestion mapping. Students conduct a simple clinical diagnostic experiment in order to identify a diseased patient.
UOM: 1 * 1 KIT


Fornecedor: Quantabio
Descrição: AccuStart™ II <i>Taq</i> DNA Polymerase is a high purity, recombinant <i>Taq</i> DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified <i>Taq</i> DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart™ II automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. The included 10X PCR Buffer II is a new optimised buffer that provides higher product yield, improved specificity, and enhanced multiplexing capability.
Fornecedor: Quantabio
Descrição: AccuStart™ II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb followed by analysis on agarose gels. It contains all components, except primers and template. AccuStart™ II GelTrack PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. The supermix includes electrophoresis tracking dyes that migrate at approximately 4 kb and 50 bp to allow direct loading of PCR product on agarose gels following amplification. AccuStart™ II <i>Taq</i> DNA polymerase in the master mix is inactivated with monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified <i>Taq</i> DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.
Código de Artigo: (TNB-6600-KIT)
Fornecedor: Tonbo Biosciences
Descrição: The fragmentation of genomic DNA by cellular nucleases during the later stages of apoptosis is also one of the most easily measured features of apoptotic cells. Nuclease activity generates DNA fragments ranging from ~300 bp to 50 bp in length, resulting in a typical DNA ‘laddering’ appearance when analyzed by agarose gel electrophoresis. These fragments have exposed 3’-hydroxyl (OH) ends which can be labeled with bromolated deoxyuridine triphosphates (Br-dUTP). An enzyme, terminal deoxynucleotidyl transferase (TdT), is used to catalyze the template-independent addition of Br-dUTP to the 3’-OH ends of double or single stranded DNA. This method is often called TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) or end labeling. Sites where the Br-dUTP is incorporated can then be detected with an antibody specific to BrdU. With the APO-BrdUTM Kit cells are first labeled with Br-dUTP, and then sites of incorporation are detected through staining with a FITC anti-BrdU antibody. Samples can then be analyzed via flow cytometry. Samples that are apoptotic will stain brightly with the anti-BrdU antibody due to the substantial number of exposed 3’-OH sites, while cells that are non-apoptotic will not have incorporated significant amounts of Br-dUTP and will stain dimly. The APO-BrdU Kit is shipped in one container and consists of two packages. Upon arrival one should be stored at 2-8°C and the other at -20°C.
UOM: 1 * 50 Tests


Fornecedor: FINNZYMES REAGENTS
Descrição: Direct PCR Kits save time and cost by allowing amplification of DNA directly from the source material. Direct PCR is based on Thermo Scientific Phire® Hot Start II DNA Polymerase and Phusion® Hot Start II High-Fidelity DNA Polymerase. These fusion enzymes are exceptionally fast, robust and highly tolerant of many PCR inhibitors present in unpurified sample material. This allows reliable amplification in challenging conditions where conventional DNA polymerases are completely inhibited.

Fornecedor: FINNZYMES REAGENTS
Descrição: Phusion® U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Fornecedor: Cytiva
Descrição: For PCR and other procedures requiring DNA polymerase activity at elevated temperatures.

Código de Artigo: (ICNA11EPTAG100)
Fornecedor: MP Biomedicals
Descrição: Taq-&GO™ is a ready to use optimised reaction mix for PCR.
UOM: 1 * 1 mL


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O stock para este item é limitado. Por favor, certifique-se de que efetuou o seu login para visualizar o stock disponível. Se a call ainda é exibida e precisar de ajuda, por favor, ligue para 213 600 770
O stock para este item é limitado. Por favor, certifique-se de que efetuou o seu login para visualizar o stock disponível. Se a call está visível e precisar de ajuda, por favor, ligue para 213 600 770
Este produto é sujeito a regulamentação especifica.
Em caso de encomenda, será contactado a solicitar documentação complementar necessária e/ou obrigatória (licença, autorização ou declaração de uso final) para a continuidade do pedido. Agradecemos a vossa compreensão
Este produto é sujeito a regulamentação especifica.
Em caso de encomenda, será contactado a solicitar documentação complementar necessária e/ou obrigatória (licença, autorização ou declaração de uso final) para a continuidade do pedido. Agradecemos a vossa compreensão.
Este produto está bloqueado. Para obter mais informações, contacte a VWR através do número 213 600 770.
O produto pretendido já não se encontra disponível. O produto indicado é um substituto.
Este produto encontra-se em rutura de stock. Poderá encontrar alternativas pesquisando pelo código de artigo indicado acima. Se precisar de ajuda, por favor contacte a VWR através do 213 600 770.
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