Procurou por: Cellulose+acetate

Fornecedor: Corning

Descrição: É possível escolher as membranas pretendidas para os filtros de seringa; os tipos de membrana disponíveis são: polietersulfona (PES), com baixa ligação proteica e débitos mais rápidos; acetato de celulose isento de surfactantes (SFCA), com ou sem pré-filtro, para menor ligação proteica; PTFE para resistência a produtos químicos; celulose regenerada (RC), a opção ideal para compatibilidade com DMSO; e Nylon (NY), que é hidrófilo, está isento de surfactantes e apresenta uma taxa muito baixa de extraíveis.

Certificados

Fornecedor: MP Biomedicals

Descrição: Storage: Room Temperature, desiccate, store under nitrogen.
Dimethyl Sulfoxide is a dipola, aprotic solvent. It has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal stability against depurination.
Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers, in antifreeze or hydraulic fluids, as a cryopreservative for cell cultures, oxidation of thiols and disulfides to sulfonic acids, as a PCR cosolvent to help improve yields, especially in long PCR. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly (A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols.
To prepare sterile solutions use a teflon or nylon membrane to sterile-filter the DMSO - do not use a cellulose acetate membrane.

Código de Artigo: (22184-250G-F)

Fornecedor: Merck

Descrição: Cellulose, Supelco®

UOM: 1 * 250 g

Certificados Promoção

Código de Artigo: (09906-1KG)

Fornecedor: Merck

Descrição: Cellulose, Supelco®

UOM: 1 * 1 kg

Promoção

Código de Artigo: (22197-250G-F)

Fornecedor: Merck

Descrição: Cellulose DS-0, pó adequado para cromatografia em camada fina (CCF/TLC), Supelco®

UOM: 1 * 250 g

Certificados Promoção

Código de Artigo: (K390-250G)

Fornecedor: VWR Chemicals

Descrição: Methyl cellulose, 4000 mPa.S (2% H₂O), alta pureza

UOM: 1 * 250 g

MSDS Promoção

Código de Artigo: (22199-50G-F)

Fornecedor: Merck

Descrição: Cellulose triacetate, Selectophore™ ionóforo, Supelco®

UOM: 1 * 50 g

Promoção

Código de Artigo: (22182-1KG)

Fornecedor: Merck

Descrição: Cellulose, Supelco®

UOM: 1 * 1 kg

Promoção

Fornecedor: DWK Life Sciences

Descrição: CELLine™ is a disposable, two-compartment bioreactor manufactured from optically clear virgin PS with a gas transfer bottom made of a moulded silicone membrane providing a 0,2 µm vent barrier. The compartments are separated by a 10 kDa semi-permeable cellulose acetate membrane and individually pressure tested for integrity. CELLine™ classic (CL) is ideal for laboratory scale applications using suspension cells or adherent cells in combination with microcarriers. The unit is optimised for cultivation of hybridomas and many other cell types (for example CHO, NSO, SF cells). CELLine™ adhere (AD) is specifically adapted to allow growth of anchorage-dependent cells (for example HEK, BHK, CHO cells). The CELLine™ AD bioreactor contains a woven PET matrix in the cell compartment providing an ideal surface for cell attachment.

Código de Artigo: (22183-1KG-F)

Fornecedor: Merck

Descrição: Cellulose, Supelco®

UOM: 1 * 1 kg

Promoção

Código de Artigo: (22525.296)

Fornecedor: VWR Chemicals

Descrição: Carboxymethyl cellulose sodium salt, TECHNICAL, viscosidade média

UOM: 1 * 1 kg

MSDS Certificados Promoção

Fornecedor: MP Biomedicals

Descrição: Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Fornecedor: VWR Collection

Descrição: Typical applications are clarification, sterile filtration, sample preparation and sterile venting.

Certificados

Código de Artigo: (298944N)

Fornecedor: VWR Chemicals

Descrição: Methyl cellulose, 300 - 560 mPa.s, Methocel® A4C

UOM: 1 * 500 g

MSDS Certificados Promoção

Fornecedor: Thermo Fisher Scientific

Descrição: Methyl cellulose, 400 cP (2% H₂O)

MSDS

Fornecedor: Thermo Fisher Scientific

Descrição: Methyl cellulose, 400 cP (C=2%, H₂O)

MSDS Certificados