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Procurou por: Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.


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Imagem do Produto-PIER88870

Cell-free protein expression vectors, T7, mammalian

Fornecedor: Thermo Fisher Scientific
Descrição: The T7 cell-free expression vectors (pT7CFE1) are cloning plasmids optimised for use with the Thermo Scientific™ 1-Step Human In Vitro Protein Expression System. They are used for the<i> in vitro</i> translation (IVT) of genes into functional proteins when used in combination with the 1-Step Human IVT system. The pT7CFE1 vector is available with single or tandem affinity tags at the N- or C- terminus to facilitate protein purification and detection. The pT7CFE vectors are suitable for insertion of cloned genes, cDNAs, ORFs or PCR products for<i> in vitro</i> transcription and translation.

Imagem do Produto-OZBIPL00130

Transfection control plasmids, pVectOZ

Fornecedor: OZ BIOSCIENCES
Descrição: pVectOZ transfection plasmids are engineered in an optimised backbone and are ideal for all transfections.

Image Unavailable-69660-3

pET-14b DNA, Novagen®

Código de Artigo: (69660-3)
Fornecedor: Merck Millipore (Novagen)
Descrição: The pET-14b vector carries an N-terminal His·Tag® sequence followed by a thrombin cleavage site and three cloning sites.
UOM: 1 * 10 µG
Image Unavailable-69752-3

Vector, pET-24d(+) DNA

Código de Artigo: (69752-3)
Fornecedor: Merck Millipore (Novagen)
Descrição: The pET-24a-d(+) vectors carry an N-terminal T7·Tag® sequence plus an optional C-terminal His·Tag® sequence.
UOM: 1 * 10 µG

MSDS


Image Unavailable-69772-3

Vector, pET-24(+) DNA

Código de Artigo: (69772-3)
Fornecedor: Merck Millipore (Novagen)
Descrição: pET-24(+) is a transcription vector designed for expression from bacterial translation signals carried within a cloned insert. It lacks the ribosome binding site and ATG start codon present on the pET translation vectors. A C-terminal His·Tag® sequence is available
UOM: 1 * 10 µG
Image Unavailable-ORIGMG225346

Slc7a11 (NM_011990) mouse tagged ORF clone

Código de Artigo: (ORIGMG225346)
Fornecedor: OriGene
Descrição: Slc7a11 (GFP-tagged) - Mouse solute carrier family 7 (cationic amino acid transporter y+ system) member 11 (Slc7a11), (10 µg).
UOM: 1 * 10 µG
Imagem do Produto-70556-3

Expression vectors, pET-41 DNA, Novagen®

Fornecedor: Merck Millipore (Novagen)
Descrição: pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand.

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pET-30a(+) DNA, Novagen®

Código de Artigo: (69909-3)
Fornecedor: Merck Millipore (Novagen)
Descrição: The pET-30a-c(+) vectors carry an N-terminal His Tag®/thrombin/S Tag™/enterokinase configuration plus an optional C-terminal His tag sequence.
UOM: 1 * 10 µG
Image Unavailable-70595-3

pSTBlue-1 AccepTor™ vector kit

Fornecedor: Merck Millipore (Novagen)
Descrição: The pSTBlue-1 AccepTor™ vector kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (eg. KOD XL polymerase and native and recombinant <i>Taq</i> polymerases), which leave single 3'-dA overhangs on the reaction products. The AccepTor™ vectors enable direct ligation by virtue of single 3'-dU overhangs that anneal efficiently with 3'-dA extensions on PCR products. The dU residues are converted to dT residues<i> in vivo</i> following transformation.

Imagem do Produto-69750-3

pET-24b(+) DNA, Novagen®

Código de Artigo: (69750-3)
Fornecedor: Merck Millipore (Novagen)
Descrição: The pET-24a-d(+) vectors carry an N-terminal T7-Tag® sequence plus an optional C-terminal His-Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs ampicillin resistance).
UOM: 1 * 10 µG
Image Unavailable-28-9546-53

Expression vectors, pGEX

Fornecedor: Cytiva
Descrição: The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.

Imagem do Produto-27-1524-01

M13KO7 Helper Phage, Cytiva

Código de Artigo: (27-1524-01)
Fornecedor: Cytiva
Descrição: M13 phage for the GST expression system.
UOM: 1 * 100 µl
Imagem do Produto-PIER16152

Expression vectors, green renilla Luc vector for luciferase assays, Pierce™

Código de Artigo: (PIER16152)
Fornecedor: Thermo Fisher Scientific
Descrição: Green <i>Renilla</i> Luc vectors contain a gene cloned from the sea pansy, <i>Renilla reniformis</i>
UOM: 1 * 10 µG
Imagem do Produto-PIER16190

Pierce™ Gaussia-Dura Luc Vector for Luciferase Assays, Expression Vectors

Fornecedor: Thermo Fisher Scientific
Descrição: <i>Gaussia</i> Dura Luc vectors contain a mutant form of the <i>Gaussia</i> luciferase gene that confers better bioluminescent signal stability than native luciferase. <i>Gaussia</i>-Dura luciferase (approx. 20 kDa) is a secreted protein, which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

Imagem do Produto-27-1542-01

E. coli BL21

Código de Artigo: (27-1542-01)
Fornecedor: Cytiva
Descrição: The <i>E. coli </i>BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.
UOM: 1 * 1 Vial
Image Unavailable-ICNA04821225

Vector, pBR322, MP Biomedicals

Código de Artigo: (ICNA04821225)
Fornecedor: MP Biomedicals
Descrição: pBR322 is a 4363 bp vector purified from <i>Escherichia coli</i>. It is one of the most commonly used cloning vectors, that confers resistance to ampicillin and tetracycline. It contains single restriction sites for Pst I and Pvu I within the ampicillin-resistance gene (ampr) and single restriction sites for BamH I, Sal I, Sph I, EcoR V, Nru I and Xma III within the tetracycline-resistance gene (tetr).
UOM: 1 * 25 µG
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O stock para este item é limitado. Por favor, certifique-se de que efetuou o seu login para visualizar o stock disponível. Se a call ainda é exibida e precisar de ajuda, por favor, ligue para 213 600 770
O stock para este item é limitado. Por favor, certifique-se de que efetuou o seu login para visualizar o stock disponível. Se a call está visível e precisar de ajuda, por favor, ligue para 213 600 770
Este produto é sujeito a regulamentação especifica.
Em caso de encomenda, será contactado a solicitar documentação complementar necessária e/ou obrigatória (licença, autorização ou declaração de uso final) para a continuidade do pedido. Agradecemos a vossa compreensão
Este produto é sujeito a regulamentação especifica.
Em caso de encomenda, será contactado a solicitar documentação complementar necessária e/ou obrigatória (licença, autorização ou declaração de uso final) para a continuidade do pedido. Agradecemos a vossa compreensão.
Este produto está bloqueado. Para obter mais informações, contacte a VWR através do número 213 600 770.
O produto pretendido já não se encontra disponível. O produto indicado é um substituto.
Este produto encontra-se em rutura de stock. Poderá encontrar alternativas pesquisando pelo código de artigo indicado acima. Se precisar de ajuda, por favor contacte a VWR através do 213 600 770.
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