Procurou por: Thermo Fisher Scientific

Descrição: Pierce™ immobilised TPCK trypsin is a serine endoprotease applicable to amino acid analysis and protein sequencing, mapping and structural studies. The immobilised form allows sample separation after treatment.

Código de Artigo: PIER20230

UOM: 1 * 2 mL

Fornecedor: Thermo Fisher Scientific

Promoção

Descrição: Surfact-Amps™ Detergent sampler is a collection of various Surfact-Amps™ detergent solutions and selected other useful detergents for protein research methods.

Código de Artigo: PIER28340

UOM: 1 * 1 KIT

Fornecedor: Thermo Fisher Scientific

Promoção

Descrição: Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.

Código de Artigo: 786-792

UOM: 1 * 1 unid.

Fornecedor: G-Biosciences

Promoção

Descrição: The carbonyl-reactive Thermo Scientific™ aminoxyTMT™ (Tandem Mass Tag™) Label Reagents enable multiplexed characterisation and quantitation of carbonyl-containing biomolecules (carbohydrates, steroids, oxidised proteins) by mass spectrometry (MS).

Código de Artigo: PIER90402

UOM: 1 * 1 KIT

Fornecedor: Thermo Fisher Scientific

Promoção

Descrição: Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques. These detergents are useful as a component of cell lysis buffers or other solutions intended to extract and solubilise proteins, improve chromatography, prepare yeast spheroplasts, and reduce background in ELISA or Western blot methods.

Código de Artigo: PIER28313

UOM: 1 * 1.000 mL

Fornecedor: Thermo Fisher Scientific

Promoção

Descrição: Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.

Código de Artigo: PIER90305

UOM: 1 * 100 µG

Fornecedor: Thermo Fisher Scientific

Certificados Promoção

Descrição: Native endoproteinase Glu-C from <i>Staphylococcus aureus</i>. Serine protease that specifically hydrolyses peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α2-macroglobulin. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

Código de Artigo: 324713-50

UOM: 1 * 50 µG

Fornecedor: Merck Millipore (Calbiochem‎)

Promoção

Descrição: PopCulture® Reagent is a detergent-based concentrate that can be added directly to cultures of <i>E. coli</i> to effectively extract proteins without the need for cell harvest. Recombinant proteins can be directly screened in the crude extract, or purified by adding an affinity matrix, washing the matrix-target protein complex to remove spent culture medium and cellular contaminants, and eluting the purified protein from the matrix. The PopCulture protein purification procedure is ideally suited to high-throughput robotic processing of samples for proteomics research or any application that would benefit from the increased speed and convenience.

Código de Artigo: 71092-3

UOM: 1 * 15 mL

Fornecedor: Merck Millipore (Novagen)

Promoção

Descrição: BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozyme™ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.

Código de Artigo: 71456-4

UOM: 1 * 500 mL

Fornecedor: Merck Millipore (Novagen)

Promoção

Descrição: The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.

Código de Artigo: 71009-4

UOM: 1 * 250 mL

Fornecedor: Merck Millipore (Novagen)

Promoção

Descrição: Native endoproteinase Asp-N from <i>Pseudomonas fragi.</i> Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyses proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

Código de Artigo: 324708-2

UOM: 1 * 2 µG

Fornecedor: Merck Millipore (Calbiochem‎)

Promoção

Descrição: Protein-OUT™ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUT™ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUT™ removes inadvertent contamination with dust and keratin.

Código de Artigo: 786-680

UOM: 1 * 50 mL

Fornecedor: G-Biosciences

Promoção

Descrição: Arg-C, sequencing grade, is an endopeptidase (clostripain) that cleaves at the C-terminus of arginine residues, including the sites next to proline and it occurs also at proline.

Código de Artigo: PROMV1881

UOM: 1 * 10 µG

Fornecedor: Promega

MSDS Promoção

Descrição: FastBreak™ cell lysis reagent is provided as a 10X concentrate and contains a proprietary non ionic detergent to facilitate lysis. Recombinant proteins can be directly screened in the cell extract or purified by the addition of the appropriate affinity matrix such as the MagneHis™ protein purification system.

Código de Artigo: PROMV8572

UOM: 1 * 40 mL

Fornecedor: Promega

Promoção

Descrição: Rabbit Reticulocyte Lysate, Untreated contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors) but has not been treated with micrococcal nuclease. Untreated Lysate is used primarily for the isolation of these components and as an abundant source of endogenous globin mRNA.

Código de Artigo: PROML41510001

UOM: 1 * 1 mL

Fornecedor: Promega

Promoção

Descrição: rLys-C, MS grade, is a recombinant Lys-C expressed in <i>E. coli. </i>It cleaves at C-terminus of lysines.

Código de Artigo: PROMV1671

UOM: 1 * 15 µG

Fornecedor: Promega

Promoção