Procurou por: Reagentes de ácidos nucleicos

Fornecedor: TriLink BioTechnologies

Descrição: Engineered RNase Inhibitor is a protein to inhibit RNase activities to prevent RNA degradation in IVT.

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Fornecedor: TriLink BioTechnologies

Descrição: CleanCap® Reagent AU is designed for the co-transcriptional capping of self-replicating or self-amplifying (saRNA) to produce an optimal Cap 1.

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Fornecedor: TriLink BioTechnologies

Descrição: This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.

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Fornecedor: OMEGA BIO-TEK

Descrição: E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200 µg of plasmid DNA in less than 60 minutes. This kit combines HiBind® membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high-quality plasmid DNA. The DNase/RNase-free HiBind® DNA midi columns operate by eliminating time consuming phenol-chloroform extractions and alcohol precipitations. Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple bind-wash-elute procedure thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, <i>E.coli </i>strain, and conditions of growth, 50 ml of an overnight culture in LB medium will typically produce 200 µg of high-copy number plasmid DNA. When working with low-copy number plasmids the starting culture volume can be increased up to 100 ml. High-quality DNA is ready for immediate use in routine molecular biology applications, such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.

MSDS

Fornecedor: Quantabio

Descrição: Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

MSDS Certificados

Fornecedor: Thermo Fisher Scientific

Descrição: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Certificados

Código de Artigo: (733-2265)

Fornecedor: Quantabio

Descrição: PerfeCTa® PreAmp SuperMix is a 5X concentrated, ready to use reaction cocktail for unbiased, selected enrichment of target sequences from limited amounts of starting material for downstream gene expression profiling or targeted resequencing. It contains all components, except primers and templates. The 5X concentrated SuperMix allows addition of higher template volumes when working with low concentration samples, and/or reduced reaction volumes. Inclusion of an inert light blue tracer dye helps visualise small reaction volumes and ensure accurate pipetting.

UOM: 1 * 40 Assays

Promoção

Código de Artigo: (ENZOENZ42817)

Fornecedor: ENZO LIFE SCIENCES

Descrição: Bio-17-ATP (Biotin-17-adenosine-5’-triphosphate) can replace ATP for <i>in vitro</i> transcription reaction catalysed by T3, T7 or SP6 RNA polymerases.

UOM: 1 * 250 Nmo

Promoção

Fornecedor: TriLink BioTechnologies

Descrição: TriLink® 2'-Deoxyadenosine-5'-O-(1-Thiotriphosphate)

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Fornecedor: TriLink BioTechnologies

Descrição: TriLink® 2'-Deoxycytidine-5'-O-(1-Thiotriphosphate)

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Fornecedor: TriLink BioTechnologies

Descrição: 2',3'-Dideoxyadenosine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddATP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.

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Fornecedor: TriLink BioTechnologies

Descrição: 2'-Fluoro NTPs are being utilised in an increasing number of applications in research and new drug development. 2'-Fluoro-dCTP is incorporated in both DNA and RNA constructs to improve <em>in vivo</em> stability.

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Fornecedor: TriLink BioTechnologies

Descrição: 5-Propargylamino-2'-deoxyuridine can be enzymatically incorporated into DNA and cDNA to introduce primary amine groups. Primary amines can be labeled with NHS ester fluors and haptens for detection and immobilisation of the resulting DNA strand.

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Fornecedor: TriLink BioTechnologies

Descrição: TriLink® 7-Deaza-7-Propargylamino-2'-deoxyguanosine-5'-Triphosphate

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Fornecedor: TriLink BioTechnologies

Descrição: This nucleotide consists of an unmodified base, a deoxyribose, and a 5′ triphosphate group for synthesis of DNA molecules.

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Fornecedor: TriLink BioTechnologies

Descrição: Cyanine 5-Aminoallyluridine-5'-Triphosphate is a substrate for T7 RNA polymerase, allowing the incorporation of cyanine 5 into RNA and mRNA during <em>in vitro</em> transcription. Cyanine 5 incorporation allows for fluorescent detection of RNA and mRNA independent of protein translation.

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