Procurou por: End-point PCR Enzymes and Kits

Fornecedor: FINNZYMES REAGENTS

Descrição: Phusion® Hot Start II High-Fidelity DNA Polymerase allows the use of a wide range of primers, including those with low melting temperatures. Phusion® Hot Start II High-Fidelity DNA Polymerase is ideal for PCR applications from routine PCR to highly demanding applications, such as cloning and high throughput PCR. It incorporates a unique dsDNA-binding domain making this Pyrococcus-like proofreading DNA polymerase extremely robust, rapid, and accurate.

Fornecedor: FINNZYMES REAGENTS

Descrição: Phusion® U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Fornecedor: FINNZYMES REAGENTS

Descrição: Phusion® Green Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion® Hot Start II DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® Hot Start II DNA polymerase and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Fornecedor: MP Biomedicals

Descrição: <i>Taq</i> DNA Polymerase is a specialised thermostable enzyme isolated from the thermophilic bacterium <i>Thermus aquaticus</i>. The recombinant form of this enzyme is expressed in <i>E. coli</i>. It is highly processive, recombinant polymerase which catalyses the addition of mononucleotide units to the 3’-OH end of a primer chain. It remains functional even after prolonged incubation at 95 °C. This enzyme possesses 5’-3’ exonuclease activiy.

Fornecedor: MP Biomedicals

Descrição: Taq-&LOAD™ PCR Master Mix is an optimised 5X reaction mix for PCR containing recombinant <i>Taq</i> DNA polymerase, dNTPs, MgCl₂, and incubation and loading buffers. Also included are a compound increasing the density of the sample and a red/purple dye.

Código de Artigo: (71975-3)

Fornecedor: Merck Millipore (Novagen)

Descrição: KOD Xtreme™ Hot Start DNA Polymerase is an ultra high fidelity DNA polymerase designed for the most challenging PCR applications, including crude sample PCR, amplification of long strand and highly GC-rich DNA templates.

UOM: 1 * 200 UA

Promoção

Código de Artigo: (71007-3)

Fornecedor: Merck Millipore (Novagen)

Descrição: The NovaTaq PCR Master Mix is a ready to use 2X concentrated mixture of NovaTaq DNA polymerase, ultra-pure deoxynucleotides, and reaction buffer without magnesium chloride. The master mix simplifies the assembly of PCR reactions and offers advantages of times savings, consistency, and minimal risk of contamination. Simply add the NovaTaq PCR master mix to an equal volume containing the required amount of magnesium chloride, DNA template, and primers, and the reaction is ready for thermal cycling. The final diluted reaction contains 2,5 units of NovaTaq DNA polymerase per 100 µl. Sufficient components are included for 200 standard 50 µl (or 100×100 µl) amplification reactions.

UOM: 1 * 250 UE

Promoção

Fornecedor: MP Biomedicals

Descrição: The Taq CORE kits are designed for the amplification of specific regions of DNA using PCR. The PCR CORE Kits - Direct Loading provide <i>Taq</i> DNA Polymerase, PCR Direct Loading buffers and dNTPs necessary for amplification and subsequent loading of samples on an agarose gel.

Fornecedor: G-Biosciences

Descrição: <i>Taq</i> DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile <i>Thermus aquaticus</i>. The molecular weight of the recombinant protein is 94 kD. The <i>Taq</i> Polymerase is able to amplify DNA up to 5 kb with an elongation velocity of 0,9 to 1,2 kb/min at 70 to 75 °C. The error rate of this <i>Taq</i> Polymerase is ~2,2×10⁻⁵ nucleotide⁻¹ cycle⁻¹. <i>Taq</i> DNA polymerase catalyses the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product

Código de Artigo: (ICNA11EPTAG100)

Fornecedor: MP Biomedicals

Descrição: Taq-&GO™ is a ready to use optimised reaction mix for PCR.

UOM: 1 * 1 mL

Promoção

Fornecedor: Merck Millipore (Novagen)

Descrição: A KOD HiFi DNA Polymerase é uma enzima de proofreading única, isolada do termófilo extremo <i>Thermococcus kodakaraensis</i> KOD1, que possui capacidades de processamento e uma fidelidade superiores, permitindo uma amplificação da PCR mais rápida e mais precisa do que a alcançada com enzimas convencionais.

Fornecedor: G-Biosciences

Descrição: <i>Pfu</i> DNA Polymerase, derived from the hyperthermophilic archae <i>Pyrococcus furiosus</i>, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). <i>Pfu</i> DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that<i> Pfu</i> DNA Polymerase-generated PCR fragments will have fewer errors than <i>Taq</i>-generated PCR inserts. Using <i>Pfu</i> DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. <i>Pfu</i> DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Fornecedor: Cytiva

Descrição: For PCR and other procedures requiring DNA polymerase activity at elevated temperatures.

Código de Artigo: (25-1500-01)

Fornecedor: Cytiva

Descrição: illustra™ Hot Start Master Mix is a 2X premixed formulation that can effectively reduce non specific priming and primer-dimer formation during PCR. illustra™ Hot Start Master Mix uses a novel hot start method called primer sequestration.

UOM: 1 * 1 KIT

Promoção

Fornecedor: Cytiva

Descrição: PuReTaq RTG PCR beads and reagents ensures reliable and robust performance in both end point and RT-PCR amplifications and ensures the lowest possible levels of contaminating prokaryotic and eukaryotic nucleic acids.

MSDS

Fornecedor: Cytiva

Descrição: illustra™ Hot Start Mix, Ready-To-Go™ is a pre-formulated and pre-dispensed, freeze-dried PCR reagent mix, including high quality PuReTaq™ DNA polymerase and a hot start activator protein, for increased specificity and reproducibility of PCR amplifications. The Ready-To-Go™ beads are provided in either 0,5 or 0,2 ml tubes that are compatible with most thermal cyclers.