Procurou por: End-point PCR Enzymes and Kits

Código de Artigo: (ENZOENZPRT1010500)

Fornecedor: ENZO LIFE SCIENCES

Descrição: Increased sensitivity standard PCR for a broader range of samples, with enhanced speed, yield, and specificity.

UOM: 1 * 1 KIT

Promoção

Código de Artigo: (ENZOENZPRT1000500)

Fornecedor: ENZO LIFE SCIENCES

Descrição: Optimised standard PCR for enhanced speed, yield, and specificity.

UOM: 1 * 1 KIT

Promoção

Fornecedor: Thermo Fisher Scientific

Descrição: Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that ensures higher PCR specificity, sensitivity and yields compared to conventional hot start Taq DNA polymerases. DreamTaq™ Hot Start DNA Polymerase uses antibody based inhibition of DNA polymerase activity at ambient temperatures, thus preventing the amplification of non-specific products prior to the amplification step.

Fornecedor: FINNZYMES REAGENTS

Descrição: Phusion® Green High-Fidelity DNA Polymerase is a combination of Phusion® DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® DNA Polymerases and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Código de Artigo: (EP0282)

Fornecedor: Thermo Fisher Scientific

Descrição: <i>Taq</i> DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium <i>Thermus aquaticus</i>. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, <i>Taq</i> DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3’-end of PCR products. Native <i>Taq</i> DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in <i>E.coli</i>.

UOM: 1 * 1 KIT

Promoção

Fornecedor: Thermo Fisher Scientific

Descrição: Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mix is a ready to use 2X reaction mix that includes DreamTaq™ Hot Start DNA Polymerase, DreamTaq™ buffer, magnesium, and dNTPs. The master mix allows easy reaction setup with fewer pipetting steps, with only primers, template, and water needing to be added. The green master mix includes density reagent and two tracking dyes for convenient direct loading of PCR products on a gel.

Fornecedor: FINNZYMES REAGENTS

Descrição: Phusion® Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion® Hot Start II High-Fidelity DNA Polymerase is resistant to main PCR inhibitors present in blood and retains its activity at blood concentrations of up to 40% in the PCR reaction.

Fornecedor: FINNZYMES REAGENTS

Descrição: Phire® Hot Start II DNA Polymerase is faster, extremely robust, and capable of amplifying long DNA fragments with high yields. Phire® Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to <i>Taq</i> DNA polymerase. In addition, the Hot Start technology allows complete reactivation of the enzyme in “zero-time” at standard cycling temperatures. This combination of features makes the polymerase an ideal solution for routine and high throughput PCR applications. Phire® Hot Start II DNA Polymerase delivers superior performance in conventional thermal cyclers as well as in fast instruments, such as the Piko® Thermal Cycler.

Fornecedor: FINNZYMES REAGENTS

Descrição: Phusion® High-Fidelity PCR Master Mix with HF Buffer is a convenient 2X mix containing Phusion® DNA Polymerase, nucleotides and optimised HF buffer including MgCl₂. Only template and primers need to be added by the user.

Fornecedor: FINNZYMES REAGENTS

Descrição: Phusion® U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Fornecedor: FINNZYMES REAGENTS

Descrição: Phire® Hot Start II DNA Polymerase is faster, extremely robust, and capable of amplifying long DNA fragments with high yields. Phire® Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to <i>Taq</i> DNA polymerase. In addition, the Hot Start technology allows complete reactivation of the enzyme in “zero-time” at standard cycling temperatures. This combination of features makes the polymerase an ideal solution for routine and high throughput PCR applications. Phire® Hot Start II DNA Polymerase delivers superior performance in conventional thermal cyclers as well as in fast instruments, such as the Piko® Thermal Cycler.

Fornecedor: FINNZYMES REAGENTS

Descrição: Direct PCR Kits save time and cost by allowing amplification of DNA directly from the source material. Direct PCR is based on Thermo Scientific Phire® Hot Start II DNA Polymerase and Phusion® Hot Start II High-Fidelity DNA Polymerase. These fusion enzymes are exceptionally fast, robust and highly tolerant of many PCR inhibitors present in unpurified sample material. This allows reliable amplification in challenging conditions where conventional DNA polymerases are completely inhibited.

Fornecedor: Merck Millipore (Novagen)

Descrição: Premixed 2X KOD Hot Start PCR components for convenience and reproducibility.

Fornecedor: Thermo Fisher Scientific

Descrição: PCR Master Mix is a 2X concentrated solution of <i>Taq</i> DNA polymerase, dNTPs and all other components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimised for efficient and reproducible PCR.

Certificados

Fornecedor: FINNZYMES REAGENTS

Descrição: Phusion® U multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

Fornecedor: FINNZYMES REAGENTS

Descrição: Phusion® high-fidelity DNA polymerases set a gold standard for high performance PCR.