Procurou por: Biology Educational Materials

Código de Artigo: (BE-404)

Fornecedor: G-Biosciences

Descrição: This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.

UOM: 1 * 1 KIT

Promoção

Código de Artigo: (BE-401)

Fornecedor: G-Biosciences

Descrição: The physical properties of proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.

UOM: 1 * 1 KIT

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Código de Artigo: (BE-502)

Fornecedor: G-Biosciences

Descrição: Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.

UOM: 1 * 1 KIT

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Código de Artigo: (EDVO124)

Fornecedor: EDVOTEK

Descrição: This experiment presents a bioterrorism scenario, with students examining a simulated DNA fingerprinting test for the detection of smallpox.

UOM: 1 * 1 unid.

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Código de Artigo: (EDVO221)

Fornecedor: EDVOTEK

Descrição: In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the <i>lacZ</i> gene. IPTG is not required in this experiment since pGAL contains the complete <i>lacZ</i> gene.

UOM: 1 * 1 unid.

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Código de Artigo: (EDVO957)

Fornecedor: EDVOTEK

Descrição: According to the World Health Organisation, between 2 and 3 million cases of skin cancer occur globally every year. Many of these cases are caused by preventable damage to DNA by UV light. In this experiment, students expose plasmid DNA to shortwave UV light to simulate the effect of sunbathing. The DNA is then analysed by agarose gel electrophoresis to observe the damage.

UOM: 1 * 1 KIT

Promoção

Código de Artigo: (EDVO990)

Fornecedor: EDVOTEK

Descrição: When normal cells are grown in culture they stop growing when they become overcrowded (contact inhibition). Cancer cells in culture grow in an uncontrolled way because they have lost this property. This helps tumours to form in the body. In addition, many different cell types can be present in a single tumour. This experiment allows students to see the differences between normal and cancer cells in both their growth and cell types.

UOM: 1 * 1 unid.

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Fornecedor: EDVOTEK

Descrição: This experiment introduces students to the use of DNA fingerprinting in a simulated paternity determination. A child's DNA fingerprint is compared with his parents.

Código de Artigo: (EDVOS-70)

Fornecedor: EDVOTEK

Descrição: The body defends itself from attack by infectious agents like bacteria and viruses by producing antibodies. Enzyme linked immunosorbent assays (ELISA) test for antibodies present in the blood, which indicate infection. In this experiment, students perform a simulated ELISA test to identify infected samples and compare them to control samples.

UOM: 1 * 1 unid.

MSDS Promoção

Código de Artigo: (EDVO303)

Fornecedor: EDVOTEK

Descrição: Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP). Bacterial cells are transformed to express the GFP. The transformed cells are then grown and the GFP purified by column chromatography. Finally, the purity of the protein fractions are analysed by SDS polyacrylamide electrophoresis.

UOM: 1 * 1 KIT

Promoção

Código de Artigo: (EDVO141)

Fornecedor: EDVOTEK

Descrição: Cancer cells differ from normal cells by the combinations of proteins that are present on their surfaces. Antibodies against these proteins will specifically bind to cancer cells and not to normal cells. This allows early detection of cancer and potentially a way of delivering cancer therapies. In this simulation experiment the reaction of cancer cell markers and their corresponding antigens are demonstrated.

UOM: 1 * 1 unid.

MSDS Promoção

Código de Artigo: (EDVO255)

Fornecedor: EDVOTEK

Descrição: When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP are used as an assay during their purification from an <i>E. coli</i> extract. The column fractions containing GFP or BFP are identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins.

UOM: 1 * 1 KIT

Promoção

Código de Artigo: (EDVO222)

Fornecedor: EDVOTEK

Descrição: The Green Fluorescent Protein (GFP) from the jellyfish <i>Aequorea victoria</i> is used extensively in all areas of science. Many organisms have been transformed with the GFP gene, the gene responsible for bioluminescence in jellyfish. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment, students transform bacteria either by GFP, BFP or both.

UOM: 1 * 1 unid.

MSDS Promoção

Código de Artigo: (763-0002)

Fornecedor: EDVOTEK

Descrição: This experiment uses PCR to amplify a small section of Lambda DNA via a 2-step process, saving valuable classroom time and allowing completion of the lab in one session.

UOM: 1 * 1 KIT

Promoção

Fornecedor: EDVOTEK

Descrição: Plasmid and lambda DNA are pre-digested with restriction endonucleases that recognise and cut double-stranded DNA within or near defined base sequences. Digests are separated by agarose gel electrophoresis.

Código de Artigo: (EDVO282)

Fornecedor: EDVOTEK

Descrição: This easy and safe experiment allows students to learn about enzyme catalysis, the nature of enzyme action, and protein structure-function relationship. Students will perform an enzyme assay and determine the rate of the enzymatic reaction.

UOM: 1 * 1 KIT

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