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Experimentation kits, introduction to ELISA reactions

Código de Artigo: (EDVO269)
Fornecedor: EDVOTEK
Descrição: Students learn the basic principles of the Enzyme-linked Immunosorbent Assay (ELISA) using this precise and sensitive antibody-based detection kit.
UOM: 1 * 1 unid.
Imagem do Produto-EDVO203

Experimentation kits, isolation of E. coli Chromosomal DNA

Código de Artigo: (EDVO203)
Fornecedor: EDVOTEK
Descrição: Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA extraction LyphoCells and reagents for isolating chromosomal DNA from <i>E. coli</i>. After spooling from solution, the DNA can be dissolved and analysed by agarose gel electrophoresis as an optional lab extension activity.
UOM: 1 * 1 KIT
Imagem do Produto-EDVO112

Ready-to-Load™ kits, analysis of Eco RI cleavage patterns of lambda DNA

Fornecedor: EDVOTEK
Descrição: Ready-to-Load™ kit for introducing the use of restriction enzymes as a tool to digest lambda DNA at specific nucleotide sequences.

Imagem do Produto-EDVO222

Transformation with blue and green fluorescent proteins

Código de Artigo: (EDVO222)
Fornecedor: EDVOTEK
Descrição: The Green Fluorescent Protein (GFP) from the jellyfish <i>Aequorea victoria</i> is used extensively in all areas of science. Many organisms have been transformed with the GFP gene, the gene responsible for bioluminescence in jellyfish. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment, students transform bacteria either by GFP, BFP or both.
UOM: 1 * 1 unid.

MSDS


Imagem do Produto-EDVO101-C

Ready-to-Load™ kits, principles and practice of agarose gel electrophoresis

Fornecedor: EDVOTEK
Descrição: Ready-to-Load™ kit to show how electrophoresis separates molecules on the basis of size and charge.

Imagem do Produto-EDVO207

Experimentation kits, southern blot analysis

Código de Artigo: (EDVO207)
Fornecedor: EDVOTEK
Descrição: This experiment introduces students to Southern blotting as a tool for DNA fingerprinting in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualised by staining.
UOM: 1 * 1 unid.
Imagem do Produto-EDVOS-68

Experimentation kits, what is an epidemic and how does an infection spread?

Código de Artigo: (EDVOS-68)
Fornecedor: EDVOTEK
Descrição: Infectious agents such as bacteria and viruses can spread rapidly through a population and cause widespready disease and death. In this experiment, students use coloured solutions to simulate the spreading of a disease in the classroom.
UOM: 1 * 1 unid.
Imagem do Produto-EDVO274

Experimentation kits, in search of the "Kissing Disease"

Código de Artigo: (EDVO274)
Fornecedor: EDVOTEK
Descrição: Infectious mononucleosis is commonly known as the 'kissing disease'. The causative agent is Epstein-Barr virus (EBV), which can be transmitted through saliva during kissing. In this experiment, students search for the presence of EBV using the ELISA reaction to detect specific viral proteins.
UOM: 1 * 1 unid.

MSDS


Imagem do Produto-EDVOS-10

What Does DNA Look Like?

Código de Artigo: (EDVOS-10)
Fornecedor: EDVOTEK
Descrição: This lab activity shows students what real chromosomal DNA looks like and allows them to explore the procedures involved in DNA extraction.
UOM: 1 * 1 unid.
Imagem do Produto-EDVO332

Experimentation kits, mitochondrial DNA analysis using PCR

Código de Artigo: (EDVO332)
Fornecedor: EDVOTEK
Descrição: The mitochondria are thought to have evolved from a symbiotic relationship between prokaryotic and eukaryotic cells. Mitochondria have their own DNA and are only inherited via the maternal line. In this experiment, students amplify two regions of their mitochondrial DNA.
UOM: 1 * 1 KIT
Imagem do Produto-EDVO300

Experimentation kits, blue/white cloning of a DNA fragment and assay of ß-galactosidase

Código de Artigo: (EDVO300)
Fornecedor: EDVOTEK
Descrição: When DNA is subcloned in the pUC polylinker region, β-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA ligase. Following the ligation to synthesis the recombinant plasmid, competent <i>E. coli</i> cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. β-galactosidase activity is assayed from blue and white bacterial cells.
UOM: 1 * 1 KIT
Imagem do Produto-EDVO302

Experimentation kits, purification of the restriction enzyme Eco RI

Código de Artigo: (EDVO302)
Fornecedor: EDVOTEK
Descrição: In this experiment, students purify the restriction enzyme, <i>Eco </i>RI. This procedure utilised an ion exchange chromatograph step for <i>Eco </i>RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by gel electrophoresis. Fractions that contain <i>Eco </i>RI are identified and pooled. The total and specific activities are calculated. Recommended for advanced courses.
UOM: 1 * 1 KIT
Imagem do Produto-EDVO117-C

Ready-to-Load™ kits, detection of mad cow disease

Fornecedor: EDVOTEK
Descrição: Bovine spongiform encephalophathy (BSE), also known as Mad Cow Disease, is a neurodegenerative, fatal condition in cattle. Consuming BSE-infected beef is believed to be the cause of a similar condition in humans, Creutzfeldt-Jakob disease. In this experiment, students examine simulated PCR products from several feed mills, to determine any possible violations of the ban on including animal parts in cattle feed.

Imagem do Produto-EDVO303

Experimentation kits, exploring biotechnology with green fluorescent protein (GFP)

Código de Artigo: (EDVO303)
Fornecedor: EDVOTEK
Descrição: Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP). Bacterial cells are transformed to express the GFP. The transformed cells are then grown and the GFP purified by column chromatography. Finally, the purity of the protein fractions are analysed by SDS polyacrylamide electrophoresis.
UOM: 1 * 1 KIT
Imagem do Produto-EDVO255

Purification and size determination of green and blue fluorescent proteins

Código de Artigo: (EDVO255)
Fornecedor: EDVOTEK
Descrição: When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP are used as an assay during their purification from an <i>E. coli</i> extract. The column fractions containing GFP or BFP are identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins.
UOM: 1 * 1 KIT
Imagem do Produto-EDVO953

Experimentation kits, Water Quality Testing III: Multiplex PCR testing of water contaminants

Código de Artigo: (EDVO953)
Fornecedor: EDVOTEK
Descrição: Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.
UOM: 1 * 1 unid.
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O stock para este item é limitado. Por favor, certifique-se de que efetuou o seu login para visualizar o stock disponível. Se a call ainda é exibida e precisar de ajuda, por favor, ligue para 213 600 770
O stock para este item é limitado. Por favor, certifique-se de que efetuou o seu login para visualizar o stock disponível. Se a call está visível e precisar de ajuda, por favor, ligue para 213 600 770
Este produto é sujeito a regulamentação especifica.
Em caso de encomenda, será contactado a solicitar documentação complementar necessária e/ou obrigatória (licença, autorização ou declaração de uso final) para a continuidade do pedido. Agradecemos a vossa compreensão
Este produto é sujeito a regulamentação especifica.
Em caso de encomenda, será contactado a solicitar documentação complementar necessária e/ou obrigatória (licença, autorização ou declaração de uso final) para a continuidade do pedido. Agradecemos a vossa compreensão.
Este produto está bloqueado. Para obter mais informações, contacte a VWR através do número 213 600 770.
O produto pretendido já não se encontra disponível. O produto indicado é um substituto.
Este produto encontra-se em rutura de stock. Poderá encontrar alternativas pesquisando pelo código de artigo indicado acima. Se precisar de ajuda, por favor contacte a VWR através do 213 600 770.
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