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Procurou por: Isothermal+Amplification+Reagents


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Imagem do Produto-25-6400-10

illustra™ TempliPhi™100/500 Amplification Kits

Fornecedor: Cytiva
Descrição: TempliPhi™ Kits efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 °C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 minutes of hands-on time to amplify 96 samples from bacterial colonies.

Imagem do Produto-25-6600-25

illustra™ GenomiPhi™ HY DNA amplification kits

Fornecedor: Cytiva
Descrição: The illustra™ GenomiPhi™ HY DNA Amplification Kit is part of the Phi29 DNA polymerase family of products. It contains all of the components necessary for genomic DNA preparation by isothermal strand displacement amplification. The product protocol is very simple, and provides typical yields of 40 to 50 μg DNA and average product lengths 10 kb. The starting material for GenomiPhi reactions can be purified DNA or non purified cell lysates.

Imagem do Produto-28-9642-86

illustra TempliPhi™ DNA sequencing template amplification kit

Código de Artigo: (28-9642-86)
Fornecedor: Cytiva
Descrição: TempliPhi™ Kits efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 °C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 minutes of hands-on time to amplify 96 samples from bacterial colonies.
UOM: 1 * 1 KIT
Imagem do Produto-1B1409-100RXN

VWR®, Hot Start PCR-to-Gel Taq PCR master mix, 2X

Código de Artigo: (1B1409-100RXN)
Fornecedor: VWR Chemicals
Descrição: Hot Start PCR-To-Gel Taq Master Mix, 2X is a ready to use reaction cocktail that contains all required components, except primers and template DNA, for routine PCR amplification of DNA fragments up to 4 kb.
UOM: 1 * 1 KIT

MSDS


Imagem do Produto-732-3647

AccuStart™ master mixes, long range SuperMix

Fornecedor: Quantabio
Descrição: Superior sensitivity and multiplexing for DNA amplification of long targets.

Imagem do Produto-733-2562

VWR®, GC Rich TEMPase Hot start DNA polymerase and MasterMix

Fornecedor: VWR Chemicals
Descrição: VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR.

Imagem do Produto-733-2845

qScript™ XLT One-Step RT-PCR Kit

Fornecedor: Quantabio
Descrição: The qScript™ XLT One-Step RT-PCR Kit is a convenient and highly sensitive system for amplification of RNA templates up to 2 kb. cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. This system has been optimised to deliver maximum endpoint RT-PCR efficiency, sensitivity, and specificity.

Certificados

Imagem do Produto-SOLUN-2011-5

TriLink® 7-Deaza-2'-deoxyguanosine-5'-Triphosphate

Fornecedor: TriLink BioTechnologies
Descrição: This deoxyguanosine analog lacks the N7 nitrogen found in the natural guanine nucleobase. Replacement of this nitrogen atom with a carbon atom results in diminished structure of the major groove in DNA and may disrupt base stacking, but does not interfere with Watson-Crick base pairing. This is desirable for applications such as amplification and sequencing of GC-rich nucleic acid sequences.

Novo produto

Image Unavailable-ENZOENZ4242010

Single-round RNA amplification and biotin labeling system

Código de Artigo: (ENZOENZ4242010)
Fornecedor: ENZO LIFE SCIENCES
Descrição: The Single-Round RNA Amplification and Biotin Labeling System provides an optimized protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for array analysis.
UOM: 1 * 1 KIT
Imagem do Produto-ENZOENZ42671K100

CYTAG™ CGH labelling kits

Fornecedor: ENZO LIFE SCIENCES
Descrição: The CYTAG™ CGH labeling kit produces high quality data for comparative genomic hybridisation (aCGH) using as little as 0,25 µg of genomic DNA, without a need for pre-amplification.

Image Unavailable-29050.

Taq DNA polymerase, Cheetah™ HotStart

Código de Artigo: (29050.)
Fornecedor: Biotium
Descrição: Cheetah™ Taq is a chemically modified hot-start Taq DNA polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Modified using a novel modifying reagent, Cheetah™ Taq represents a major improvement upon AmpliTaq Gold by having a faster activation time and better shelf-life.
UOM: 1 * 500 UE
Imagem do Produto-733-2883

VWR®, Fast HiFi DNA Polymerase, 2 U/µl

Fornecedor: VWR Chemicals
Descrição: Fast HiFi DNA Polymerase 2 U/µl is a proof reading DNA polymerase displaying the following features; high fidelity >60X Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. lt is recommended for applications, which require extremely high fidelity such as cloning/subcloning, NGS applications and mutagenesis.

Image Unavailable-ENZOENZ424220010

BIOARRAY™ Low Input RNA Amplification with Biotin Labeling System

Código de Artigo: (ENZOENZ424220010)
Fornecedor: ENZO LIFE SCIENCES
Descrição: The BIOARRAY™ Low Input RNA Amplification with Biotin Labeling System provides an optimised protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for array analysis.
UOM: 1 * 1 KIT
Imagem do Produto-733-2885

VWR®, Fast HiFi DNA polymerase, 2X master mix

Fornecedor: VWR Chemicals
Descrição: Fast HiFi DNA Polymerase 2 U/µl is a proofreading DNA polymerase displaying the following features; high fidelity >60X Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. lt is recommended for applications, which require extremely high fidelity such as cloning / sub cloning, NGS applications and mutagenesis.

Imagem do Produto-733-2106

AccuStart™ Genotyping ToughMix®

Fornecedor: Quantabio
Descrição: AccuStart™ Genotyping ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes.

Certificados

Image Unavailable-733-2082

One-Step SYBR® Green qRT-PCR, qScript™

Fornecedor: Quantabio
Descrição: qScript™ One-Step SYBR® Green qRT-PCR Kits reliably deliver maximum qRT-PCR efficiency, sensitivity and specificity in with a simplified protocol. These kits offer reliable and unbiased amplification of all RNA targets regardless of abundance. The proprietary supermix maximises activities of both reverse transcriptase and AccuStart™ <i>Taq</i> DNA polymerase without compromising specificity of amplification. qScript™ One-Step SYBR® Green qRT-PCR kits are available and optimised for all real-time PCR instrument platforms including those requiring normalisation with ROX reference dye.

MSDS Certificados

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O stock para este item é limitado. Por favor, certifique-se de que efetuou o seu login para visualizar o stock disponível. Se a call ainda é exibida e precisar de ajuda, por favor, ligue para 213 600 770
O stock para este item é limitado. Por favor, certifique-se de que efetuou o seu login para visualizar o stock disponível. Se a call está visível e precisar de ajuda, por favor, ligue para 213 600 770
Este produto é sujeito a regulamentação especifica.
Em caso de encomenda, será contactado a solicitar documentação complementar necessária e/ou obrigatória (licença, autorização ou declaração de uso final) para a continuidade do pedido. Agradecemos a vossa compreensão
Este produto é sujeito a regulamentação especifica.
Em caso de encomenda, será contactado a solicitar documentação complementar necessária e/ou obrigatória (licença, autorização ou declaração de uso final) para a continuidade do pedido. Agradecemos a vossa compreensão.
Este produto está bloqueado. Para obter mais informações, contacte a VWR através do número 213 600 770.
O produto pretendido já não se encontra disponível. O produto indicado é um substituto.
Este produto encontra-se em rutura de stock. Poderá encontrar alternativas pesquisando pelo código de artigo indicado acima. Se precisar de ajuda, por favor contacte a VWR através do 213 600 770.
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