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Imagem do Produto-BE-404

Experimentation kits, hydrophobic and hydrophilic proteins

Código de Artigo: (BE-404)
Fornecedor: G-Biosciences
Descrição: This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.
UOM: 1 * 1 KIT
Imagem do Produto-BE-401

Experimentation kits, physical properties of proteins

Código de Artigo: (BE-401)
Fornecedor: G-Biosciences
Descrição: The physical properties of proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.
UOM: 1 * 1 KIT
Imagem do Produto-BE-409

Experimentation kits, conservation of genetic information

Código de Artigo: (BE-409)
Fornecedor: G-Biosciences
Descrição: In this lab activity, students learn to perform carefully controlled experiments to generate protein fragments using a proteolysis enzyme and then analyse the protein fragments by electrophoresis. By analysis of protein fragmentation patterns, i.e. protein fingerprints, students learn about protein sequence, structure, and their conservation. Students resolve a set of 3 functionally identical protein samples selected from throughout the animal kingdom; including human, bovine and sheep. After generating fingerprints by electrophoresis, students examine the protein fingerprint of each sample to determine the degree of conservation.
UOM: 1 * 1 KIT
Imagem do Produto-EDVO282

Experimentation kits, principles of enzyme catalysis

Código de Artigo: (EDVO282)
Fornecedor: EDVOTEK
Descrição: This easy and safe experiment allows students to learn about enzyme catalysis, the nature of enzyme action, and protein structure-function relationship. Students will perform an enzyme assay and determine the rate of the enzymatic reaction.
UOM: 1 * 1 KIT
Imagem do Produto-BE-318

Experimentation kits, nucleic acid quantification

Código de Artigo: (BE-318)
Fornecedor: G-Biosciences
Descrição: The kit utilises the principle of diffusion of nucleic acids on a nylon membrane to determine their concentration. No spectrophotometers required.
UOM: 1 * 1 KIT
Imagem do Produto-BE-408

Experimentation kits, protein fingerprinting

Código de Artigo: (BE-408)
Fornecedor: G-Biosciences
Descrição: In this lab activity, students learn to perform simple protein isolation procedures to isolate the protein fingerprint from various fresh tissues. They will compare the protein fingerprints of 4 different tissues to understand that the function of a particular organ is due to the proteins that are localised to the specific organ. Also included in this kit are four dried protein samples (from mouse liver, brain, heart and lungs) to compare as a control, if fresh tissues cannot be obtained.
UOM: 1 * 1 KIT
Imagem do Produto-BE-312

Experimentation kits, expression of a recombinant protein

Código de Artigo: (BE-312)
Fornecedor: G-Biosciences
Descrição: The final goal in molecular biology is often the expression of a recombinant protein. The transformed plasmids can be used as templates by the bacteria to produce protein. Students learn about essential promoters and other elements necessary for successful protein expression in bacteria, including the differences between inducible and constitutive (unregulated) expression.The Expression of a Recombinant Protein kit allows students to express a protein either constitutively or under the control of an inducible promoter, which is activated with IPTG (isopropyl-beta-D-thiogalactopyranoside).
UOM: 1 * 1 KIT
Imagem do Produto-EDVO203

Experimentation kits, isolation of E. coli Chromosomal DNA

Código de Artigo: (EDVO203)
Fornecedor: EDVOTEK
Descrição: Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA extraction LyphoCells and reagents for isolating chromosomal DNA from <i>E. coli</i>. After spooling from solution, the DNA can be dissolved and analysed by agarose gel electrophoresis as an optional lab extension activity.
UOM: 1 * 1 KIT
Imagem do Produto-EDVO302

Experimentation kits, purification of the restriction enzyme Eco RI

Código de Artigo: (EDVO302)
Fornecedor: EDVOTEK
Descrição: In this experiment, students purify the restriction enzyme, <i>Eco </i>RI. This procedure utilised an ion exchange chromatograph step for <i>Eco </i>RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by gel electrophoresis. Fractions that contain <i>Eco </i>RI are identified and pooled. The total and specific activities are calculated. Recommended for advanced courses.
UOM: 1 * 1 KIT
Imagem do Produto-EDVO202

Experimentation kits, Mini-Prep isolation of plasmid DNA

Código de Artigo: (EDVO202)
Fornecedor: EDVOTEK
Descrição: Small-scale, rapid isolation of plasmid DNA is a routine procedure used for screening and analysis of recombinant DNAs in cloning and sub-cloning experiments. In this experiment, students isolate plasmid DNA without the use of toxic chemicals such as phenol or chloroform.
UOM: 1 * 1 KIT
Imagem do Produto-EDVOS-47

Linking food science to biotechnology kit

Código de Artigo: (EDVOS-47)
Fornecedor: EDVOTEK
Descrição: Investigate how agarose gel electrophoresis unlocks the color code used by food scientists to make colorful candies.
UOM: 1 * 1 KIT
Imagem do Produto-EDVO266

What's in my lunch? quantitative milk allergy ELISA kit

Código de Artigo: (EDVO266)
Fornecedor: EDVOTEK
Descrição: Milk proteins are the most common food allergens in children. Accurate detection and labeling is vital to inform consumers about potentially dangerous foods.
UOM: 1 * 1 KIT
Imagem do Produto-EDVO235

Experimentation kits, DNA/RNA microarrays

Código de Artigo: (EDVO235)
Fornecedor: EDVOTEK
Descrição: Microarray technology allows scientists to screen large numbers of samples in one assay. This technology allows high-throughput screening of small samples and allows comparisons to be made between healthy and unhealthy tissues. In this experiment, students apply simulated DNA and RNA samples to a membrane to screen for positive and negative samples.
UOM: 1 * 1 KIT
Imagem do Produto-BE-407

Experimentation kits, protein structure analysis

Código de Artigo: (BE-407)
Fornecedor: G-Biosciences
Descrição: This lab activity has two objectives - to deepen the understanding of protein molecules and for students to learn the potential of electrophoresis in protein analysis. Students study the fundamentals of protein structure from their primary structure to the more complex tertiary and quaternary structures, utilising protein electrophoresis. Complex mixtures of protein samples and characterised pure protein samples, in conjunction with electrophoresis, are utilised to study protein structure and the potential of protein electrophoresis. Using non-denaturing and denaturing electrophoresis, students understand the difference between primary, tertiary and quaternary structures, the importance of disulfhydryl bridges in maintaining protein structure and electrophoresis in studying complete proteins and protein subunits.
UOM: 1 * 1 KIT
Imagem do Produto-BE-314

Experimentation kits, mutation detection and analysis

Código de Artigo: (BE-314)
Fornecedor: G-Biosciences
Descrição: A major challenge for molecular biologists and genetic engineers is to easily detect and analyze genetic mutations that occur naturally, causing diseases, or during genetic engineering or cloning, whether deliberate or accidental. Students learn about different types of genetic mutations, including substitutions, deletions and point mutations and about various techniques used to detect genetic mutations. The kit contains all the reagents to screen simulated clinical samples for a mutant gene, using both the polymerase chain reaction (PCR) and restriction digestion mapping. Students conduct a simple clinical diagnostic experiment in order to identify a diseased patient.
UOM: 1 * 1 KIT
Imagem do Produto-BE-405

Experimentation kits, enzyme analysis

Código de Artigo: (BE-405)
Fornecedor: G-Biosciences
Descrição: This lab activity involves analysis of an enzyme reaction using a specific enzyme substrate and inhibitor. Students study how the rate of enzyme reaction is dependent on substrate concentration and the influence of agents (inhibitors) and conditions that affects the enzyme reaction such as pH and temperature.
UOM: 1 * 1 KIT
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O stock para este item é limitado. Por favor, certifique-se de que efetuou o seu login para visualizar o stock disponível. Se a call ainda é exibida e precisar de ajuda, por favor, ligue para 213 600 770
O stock para este item é limitado. Por favor, certifique-se de que efetuou o seu login para visualizar o stock disponível. Se a call está visível e precisar de ajuda, por favor, ligue para 213 600 770
Este produto é sujeito a regulamentação especifica.
Em caso de encomenda, será contactado a solicitar documentação complementar necessária e/ou obrigatória (licença, autorização ou declaração de uso final) para a continuidade do pedido. Agradecemos a vossa compreensão
Este produto é sujeito a regulamentação especifica.
Em caso de encomenda, será contactado a solicitar documentação complementar necessária e/ou obrigatória (licença, autorização ou declaração de uso final) para a continuidade do pedido. Agradecemos a vossa compreensão.
Este produto está bloqueado. Para obter mais informações, contacte a VWR através do número 213 600 770.
O produto pretendido já não se encontra disponível. O produto indicado é um substituto.
Este produto encontra-se em rutura de stock. Poderá encontrar alternativas pesquisando pelo código de artigo indicado acima. Se precisar de ajuda, por favor contacte a VWR através do 213 600 770.
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