Procurou por: Western Blotting Reagents

Código de Artigo: (PIER16180)

Fornecedor: Thermo Fisher Scientific

Descrição: Pierce™ Firefly Signal Enhancer (100X) is designed to complement the Firefly Luciferase Flash and Glow assay and is recommended when maximum sensitivity is desired.

UOM: 1 * 0,5 mL

Promoção

Código de Artigo: (PIER32110)

Fornecedor: Thermo Fisher Scientific

Descrição: Miser™ Antibody Extender Solution NC is a post-transfer nitrocellulose membrane-treatment reagent that enables Western blot detection with much less primary antibody.

UOM: 1 * 500 mL

Promoção

Fornecedor: G-Biosciences

Descrição: Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis. The presence of contaminants, or interfering agents, such as salts, acids, bases and detergents, result in band distortion and poor protein resolution. As a result, SDS-PAGE gels are hard to analyse and lack reproducibility. PAGE-Perfect™ is a simple, two-step method for concentrating, cleaning and preparing protein solutions for running publication quality gels.

Código de Artigo: (69078-3)

Fornecedor: Merck Millipore (Novagen)

Descrição: The Perfect Protein™ HRP Western Blot Kit includes an HRP or AP conjugate in addition to Perfect Protein™ Western markers.

UOM: 1 * 1 unid.

Promoção

Fornecedor: Thermo Fisher Scientific

Descrição: SSC solution is commonly used in nucleic acid hybridization techniques at concentrations ranging from 0,1X to 20X depending on the application. It is used to facilitate transfer of nucleic acids to membranes in Southern and Northern blotting, dot blotting, and colony and plaque lifts.

MSDS Certificados

Fornecedor: Rockland Immunochemicals

Descrição: FemtoMax™ is an extremely sensitive, non radioactive, and enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP). It allows for the detection of femtogram (10 to 15) amounts of antigen using photographic film or other imaging methods including highly sensitive CCD cameras.

Fornecedor: Thermo Fisher Scientific

Descrição: The Fast Western Blot Kit, SuperSignal™ West Pico Substrate contains optimised reagents and protocol for picogram-level detection and and Western blot development less than 1 hour after gel transfer.

Fornecedor: Rockland Immunochemicals

Descrição: TrueBlot® Western blot kits contain the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples using TrueBlot® second step immunoblotting reagents in conjunction with the user's own primary IP antibody and primary (mouse, goat or rabbit IgG) Western blotting antibody.

Código de Artigo: (WB59-100)

Fornecedor: Merck Millipore (Oncogene)

Descrição: This Western-Re-Probe™ reagent is validated for use in immunoblotting.

UOM: 1 * 100 mL

Promoção

Fornecedor: Thermo Fisher Scientific

Descrição: The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes.

Fornecedor: G-Biosciences

Descrição: Perfect-FOCUS™ is based on quantitative precipitation and concentration of protein solutions using UPPA™ (Universal Protein Precipitation Agent) and has been specifically developed for preparing lower conductivity protein samples for iso-electric focusing gels. Perfect-FOCUS™ is designed to clean and remove interfering agents from protein samples and concentrate protein solutions that give poor protein spot resolution during 2D electrophoresis.

Código de Artigo: (J63209.AK)

Fornecedor: Thermo Fisher Scientific

Descrição: Saponin permeating solution, is used as a cell permeabilising agent.

UOM: 1 * 250 mL

MSDS Certificados Promoção

Fornecedor: Thermo Fisher Scientific

Descrição: For chemiluminecent HRP-based detection of western blotting systems. It is soluble in most polar organic solvents but is insoluble in water.

MSDS Certificados

Código de Artigo: (PIER15159)

Fornecedor: Thermo Fisher Scientific

Descrição: QuantaRed™ Enhanced Chemifluorescent HRP Substrate contains chemical enhancers to increase the fluorescent yield and sensitivity of 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) chemifluorescence.

UOM: 1 * 1 unid.

Promoção

Código de Artigo: (TB110)

Fornecedor: G-Biosciences

Descrição: Kingdom blot is prepared using total protein extracted from a variety of species, where whole species is not available the whole liver lysate is used. These blots can be used to identify similar proteins in other species, including human, chicken, frog, worm, Drosophila, yeast, Aspergillus, <i>E. coli</i>, Rhizobium and green algae.

UOM: 1 * 1 unid.

Promoção

Fornecedor: Rockland Immunochemicals

Descrição: These Chemiluminescent Western Blot Kits allow for the detection of primary polyclonal or monoclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane-bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialised formulation of luminol, an extremely sensitive, non radioactive substrate that emits light and allows visualisation using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Because of the extremely high sensitivity of the kit, primary and secondary antibodies can be used at greater dilutions. Primary antibodies currently diluted 1:1000 using an ECL™ substrate is the equivalent of a 1:5000 dilution using the FemtoMax™ substrate.