Procurou por: Western Blotting Reagents

Fornecedor: Rockland Immunochemicals

Descrição: NPP ELISA alcaline phosphatase substrate is a chromogenic substrate used in immunosorbent assays.

Fornecedor: G-Biosciences

Descrição: Ready-to-screen, human, single tissue blots, from both normal and tumour tissues. G-Biosciences pre-made blots are prepared with the appropriate GenLysate™ and 50 μg protein is loaded onto a 4 to 20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved, they are transferred to a PVDF membrane, using our Western transfer buffer.

Código de Artigo: (BB01)

Fornecedor: G-Biosciences

Descrição: G-Biosciences' Botanical Garden Blot is prepared using total protein extracted from a variety of 5 to 10 day old, whole plant tissues.

UOM: 1 * 1 unid.

Promoção

Fornecedor: G-Biosciences

Descrição: G-Biosciences offers ready-to-screen, normal tissue, brain tissue region blots. The brain tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse, rat, human, and primate brains.

Fornecedor: G-Biosciences

Descrição: These are ready to screen, normal tissue, heart tissue region blots from human and pig. The heart tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult pig and adult human hearts.

Fornecedor: Merck

Descrição: The Visualizer Western Blot Detection kit is designed for the sensitive detection of proteins in chemiluminescent Western blots.

Código de Artigo: (MILF2502)

Fornecedor: Merck

Descrição: Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

UOM: 1 * 25 mL

Promoção

Fornecedor: G-Biosciences

Descrição: This blot contains two protein lanes, a protein marker lane and a lane with one GenLysate™ sample. After the proteins are resolved, they are transferred to a PVDF membrane using Western Transfer Buffer. The blots are ready to be blocked and probed with the antibodies of choice.

Fornecedor: G-Biosciences

Descrição: This blot contains two protein lanes, a protein marker lane and a lane with one GenLysate™ sample. After the proteins are resolved, they are transferred to a PVDF membrane using Western Transfer Buffer. The blots are ready to be blocked and probed with the antibodies of choice.

Código de Artigo: (MILF2060)

Fornecedor: Merck

Descrição: Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

UOM: 1 * 1 unid.

Promoção

Fornecedor: Thermo Fisher Scientific

Descrição: SuperSignal™ West Femto maximum sensitivity substrate enables detection of low-femtogram amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies.

MSDS Certificados

Fornecedor: Thermo Fisher Scientific

Descrição: Some antibodies are difficult to remove from Western blots and require Longer incubation times or incubation temperatures greater than 22 °C. Restore™ PLUS Western Blot Stripping Buffer was developed to reduce incubation times while keeping incubations at room temperature. High-affinity antibodies can be quickly and effectively stripped from Western blots without removing transferred proteins, thereby allowing multiple reprobes of the target.

Fornecedor: Thermo Fisher Scientific

Descrição: The Fast Western Blot Kit, ECL Substrate contains optimised reagents that shorten the time which is required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.

Código de Artigo: (PIER34035)

Fornecedor: Thermo Fisher Scientific

Descrição: NBT (nitro-blue tetrazolium chloride) yields a blue-purple, water-insoluble product upon reduction of the tetrazolium salt. The product of reduction is NBT-formazan. This substrate is widely used for immunochemistry applications because the color is linear and stable. NBT is used as the substrate for both glucose oxidase and alkaline phosphatase systems.

UOM: 1 * 1 g

Promoção

Fornecedor: Thermo Fisher Scientific

Descrição: Guardian™ Peroxidase conjugate stabiliser/diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long term storage.

Fornecedor: Thermo Fisher Scientific

Descrição: The SuperSignal™ West Dura Extended Duration Substrate is a sensitive, luminol-based enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. SuperSignal West Dura Substrate enables mid-femtogram detection of antigen by oxidising luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualised on X-ray film or an imaging system. Optimal signal intensity and duration can be attained with appropriate primary and secondary antibody dilutions.

MSDS