Ponceau S, bioreagent Electrophoresis, Sigma-Aldrich®
Fornecedor: SIGMA ALDRICH MICROSCOPY
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Atenção
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Sinônimos:
3-Hydroxy-4-(2-sulpho-4-[4-sulphophenylazo]phenylazo)-2,7-naphthalenedisulphonic acid sodium salt, CI 27195, Ponceau, 3-Hydroxy-4-((2-sulpho-4-((4-sulphophenyl)diazenyl)phenyl)diazenyl)naphthalene-2,7-disulphonic acid, tetrasodium salt
MQ200
Ponceau S is the sodium salt of a diazo dye. It is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection. The following are two common stain formulations: 0.1% Ponceau S (w/v) in 5% acetic acid 2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid.
- Ponceau S has been used for membrane staining (Western blotting)
Fórmula:
C₂₂H₁₂N₄Na₄O₁₃S₄ Peso Molecular: 760.56 g/mol Temperatura de Armazenamento: Room Temperature |
Número MDL :
MFCD00003892 Número CAS: 6226-79-5 EINECS: 228-319-2 |
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Especificações dos Resultados do Teste
Infrared spectrum | Conforms to Structure |
Solubility (Turbidity) | Clear |
Solubility (Colour) c = 4mg/4ml; Water |
Red to Very Dark Red |
Suitability Suitable for Use as a Stain in Electrophoresis |
Suitable |
Wavelength C = 0.015 G/L, Di water |
518 - 522 nm |
Extinction Coefficient | ≥33000 |
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