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Glycogen-Glo™ Glycogen Assay Kits, Easy, Sensitive

Fornecedor: Promega

J5052 J5051
PROMJ5052EA 3890 EUR
PROMJ5052 PROMJ5051
Glycogen-Glo™ Glycogen Assay Kits, Easy, Sensitive
Ensaios Ensaios celulares

The Glycogen-Glo™ Assay is a fast and sensitive method for detecting glycogen from various biological samples, such as cultured cells and tissue homogenates.


  • Efficient glycogen detection from diverse samples
  • Compatible with cell lysates and tissue homogenates
  • In well sample preparation protocol streamlines workflow
  • Scalable to 384 well plates for HTS applications
  • Linear range: 20 µg/ml glycogen
  • Maximum assay window: >150


Based on bioluminescent technology, the Glycogen-Glo™ Assay can detect subtle changes in glycogen synthesis, storage and breakdown. Its streamlined sample preparation protocol involves acid treatment and neutralization directly in the assay wells, eliminating the need for cell collection, centrifugation and spin columns. The assay is adaptable to a 384-well format, facilitating quick analysis of many samples.


The Glycogen-Glo™ Assay utilizes a series of enzyme-coupled reactions. First, glycogen is digested into glucose using glucoamylase enzyme. The resulting glucose is measured using glucose dehydrogenase in conjunction with a bioluminescent NADH detection technology. The result is a light signal proportional to the starting glycogen concentration in the sample.


Samples that contain both glycogen and glucose require two reactions to determine the glycogen concentration. One reaction is used to measure total glucose resulting from digested glycogen plus any glucose present in the sample. The second is used to measure any glucose that was present in the sample prior to digestion. The difference in signal between the two reactions represents the contribution from glycogen in the sample. The effective digestion of glycogen into glucose monomers (≥70%) simplifies data interpretation and calculations.


Cellular glycogen content varies greatly depending on cell type and growth conditions. The high sensitivity and wide linearity of the Glycogen-Glo™ Assay accommodate measurement of glycogen in cells, regardless of whether they have low or high glycogen levels. The data show analysis of intracellular glycogen content of three cell lines using the Glycogen-Glo™ Assay.

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