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L(+)-Ramnose monohidratada

Fornecedor: Promega

Sinônimos: (2R,3R,4S,5S)-2,3,4,5-tetrahydroxyhexanal hydrate, 6-Deoxy-L(+)-mannose monohydrate

L5701 L5702
PROML5701EA 91.4 EUR
PROML5701 PROML5702
L(+)-Ramnose monohidratada
L(+)-Ramnose monohidratada

nduces Expression of ORFs in Vectors Transformed into Single Step (KRX) E. coli Cells


  • Used with Single Step (KRX) Competent Cells
  • Provides dramatic control of expressed protein-coding regions with higher protein yields
  • Induces expression of T7 RNA Polymerase under control of rhamnose promoter rhaBAD


The Single Step (KRX) Competent Cells are designed for efficient transformation and tightly controlled protein expression. These cells consolidate the best attributes of these two steps into one strain to evaluate protein expression in E. coli. In two days, you can transform your vector into the Single Step (KRX) Competent Cells and be ready for protein expression. Transformation efficiencies are greater than 108 cfu/μg, similar to other highly competent cells. The single step cells are available in single transformation size (50μl). KRX also can be used for blue/white screening. Single Step (KRX) is an E. coli K strain that contains a chromosomal copy of the T7 RNA polymerase driven by a rhamnose promoter (rhaBAD) to provide dramatic control of the proteins expressed via a T7 promoter. Pre-induced expression protein levels are significantly lower than those of BL21(DE3)-derived strains. This feature facilitates cloning and expression of proteins toxic to E. coli.

Genotype: [F´, traD36, ΔompP, proA+B+, lacIq, Δ(lacZ)M15] ΔompT, endA1, recA1, gyrA96 (Nalr), thi-1, hsdR17 (rk–, mk+), e14– (McrA–), relA1, supE44, Δ(lac-proAB), Δ(rhaBAD)::T7 RNA polymerase.
Stotage: 15°C to 30°C

Fórmula: C₆H₁₂O₅·1H₂O
Ponto de Fusão: 85…95 °C
Densidade: 1,47 g/cm³ (20 °C)
Temperatura de Armazenamento: Ambiente
Número MDL : MFCD00149363
Número CAS: 10030-85-0
EINECS: 222-793-4
Merck Index: 12,08338

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