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Violeta de cristal solução aquosa etanólica coloração primária para coloração de Gram, Sigma-Aldrich®

Fornecedor: Merck
Atenção

Sinônimos: Cloreto de 4-[4,4'-bis(dimetilamino)-benzidrilideno]ciclohexa-2,5-dien-1-ilideno]dimetilamónio, CI 42555

1.09218.0500 1.09218.2500
1.09218.0500EA 40.5 EUR
1.09218.0500 1.09218.2500
Violeta de cristal solução aquosa etanólica coloração primária para coloração de Gram, Sigma-Aldrich®
Violeta básico nº 3

The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative.


The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by safranin solution and appear orange.

Fórmula: C₂₅H₃₀ClN₃
Temperatura de Armazenamento: Ambiente
Número MDL : MFCD00011750
Número CAS: 548-62-9
UN: 1170
ADR: 3,III
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