RNA purification, ReliaPrep™ RNA Miniprep kit
Fornecedor: Promega
ReliaPrep™ RNA Miniprep systems provide a fast and simple technique for preparation of intact total RNA from cultured cells or tissue. The proprietary column/binding matrix efficiently captures RNA from very small amounts of input material, isolating RNA eluted in a minimal volume (less than 15 µl).
- Simple 30 minute protocol
- Elutes in small volume, generating highly concentrated RNA
- No phenol/chloroform or ß-mercaptoethanol required
Using this membrane-based purification system, from 100 to 5×10⁶ cultured cells or 0,25 to 20 mg of tissue can be processed per purification. The system incorporates a DNase treatment step directly on the minicolumn membrane and effectively removes substances that can inhibit downstream assays.
Fornecimento: Kit contains ReliaPrep™ minicolumns; collection tubes; elution tubes; BL buffer; column wash solution (CWE); DNase I (lyophilised); MnCl₂ (0,09 M); yellow core buffer; RNA wash solution (RWA); 1-Thioglycerol; nuclease-free water.
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