Cell signaling assays, ProFluor® Src-Family Kinase Assay
Fornecedor: Promega
ProFluor® Src-Family Kinase Assay measures the activity of purified Src-family tyrosine kinases (Src, Lck, Lyn, Fyn, and Hck tested) in a multiwell plate format and involves 'add-mix-read' steps only. Rhodamine 110 fluorescent signal produced is much higher than the fluorescent signal given off by test compounds.
- Ideal for high-throughput phosphatase assays in 96- or 384-well plate formats
- Minimal test compound interference
- Control peptide included (AAF-AMC) for monitoring protease activity, reducing false-positive hits
- No radioactive waste disposal and safety issues
ProFluor® Src-Family Kinase Assay produces excellent Z´ values (>0,7) in either 96- or 384-well plate formats and easily distinguishes known Src-family kinase inhibitors from other compounds.
Fornecimento: Kit includes stabiliser reagent, protease reagent, Src-family kinase R110 substrate, control AMC substrate, MnCl₂, rATP (10 mM), Sodium vanate (100 mM), Reaction buffer B (5X), Termination buffer B (5X).
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