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Cell signaling assays, ProFluor® Ser/Thr PPase Assay

Fornecedor: Promega

V1260 V1261
PROMV1260EA 0 EUR
PROMV1260 PROMV1261
Cell signaling assays, ProFluor® Ser/Thr PPase Assay
Ensaios Ensaios celulares

ProFluor® Ser/Thr PPase Assay measures purified serine/threonine protein phosphatase activity in a multiwell plate format and involves 'add-mix-read' steps only. The assay works with protein phosphatase 1 (PP1), PP2A, PP2B and PP2C.


  • Ideal for high-throughput phosphatase assays in 96- or 384-well plate formats
  • Minimal test compound interference
  • Control peptide included (AAF-AMC) for monitoring protease activity, reducing false-positive hits
  • No radioactive waste disposal and safety issues


ProFluor® Ser/Thr PPase Assay produces excellent Z´ values (>0,8) in either 96- or 384-well plate formats and easily distinguishes known phosphatase inhibitors from other compounds.


Fornecimento: Kit includes stabiliser reagent, protease reagent, S/T PPase R110 substrate, control AMC substrate, MgCl₂, MnCl₂, CaCl₂, NiCl₂, Reaction buffer B (5X), Termination buffer B (5X).

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