RNA isolation system, SV total RNA
Fornecedor: Promega
The SV Total RNA Isolation System provides a fast and simple technique for preparation of intact total RNA from tissues, cultured cells and white blood cells in as little as one hour.
- Rapid isolation of high yields of total RNA without the use of hazardous compounds
- Choice of microcentrifugation (spin) or vacuum purification methods
- Purified RNA suitable for all routine molecular biology applications, including RT-PCR
SV Total RNA Isolation System incorporates a DNase treatment step directly on the minicolumn membrane. This step substantially reduces genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without the use of phenol:chloroform extractions or ethanol precipitations, and there is no DNase carry over in the final RNA preparation.
Fornecimento: Kit includes collection tubes; elution tubes; spin columns; RNA lysis buffer (RLA); RNA dilution buffer (RDA) (blue buffer); β-mercaptoethanol (97,4%); DNase I (lyophilised); MnCl₂ (0,09 M); yellow core buffer; DNase stop solution (DSA) (concentrated); RNA wash solution (RWA) (concentrated); nuclease-free water.
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