Large scale RNA production systems, RiboMAX™
Fornecedor: Promega
The RiboMAX™ large scale RNA production systems consistently produce 2 to 5 mg/ml of RNA in a 1 ml reaction, about 10- to 20-fold more RNA than is produced with the standard Riboprobe® System transcription reaction.
- Available for use with SP6 or T7 RNA polymerase
- Scalable to meet varying RNA production requirements
RNAs synthesised with the RiboMAX™ system perform better for in vitro translation in rabbit reticulocyte translation systems than RNA synthesised by standard methods. The reduction of components inhibitory to translation may be an advantage for other applications requiring biologically active RNA. Because the RiboMAX™ systems produce large quantities of RNA, they are not recommended for generating high specific activity RNA probes.
Applications:
Generation of large quantities of capped or uncapped RNA transcripts for in vitro or in vivo translation.
In vitro synthesis of tRNA, rRNA, RNA virus genomes and ribozymes.
Production of substrates for RNA splicing, RNA secondary structure, antisense RNA and RNA:protein interaction studies.
Synthesis of long RNAs for nonmammalian RNA interference.
Note: Use of the RiboMAX™ System for production of capped transcripts requires separate purchase of the Ribo m⁷G Cap Analog.
Fornecimento: Includes rATP, 100 mM, rUTP, 10 0mM, rGTP, 100 mM, rCTP, 100 mM, 5X SP6 Transcription Buffer, SP6 Linear Control DNA, Sodium Acetate, 3,0 M (pH 5,2), Enzyme Mix, SP6 RNA Polymerase, Nuclease-Free Water and RQ1 RNase-Free DNase.
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