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Recombinant terminal deoxynucleotidyl transferase

Fornecedor: Promega

M1871 M1875
PROMM18710300EA 119 EUR
PROMM18710300 PROMM18751500
Recombinant terminal deoxynucleotidyl transferase
Enzimas

Terminal deoxynucleotidyl transferase, recombinant, catalyses the repetitive addition of mononucleotides to the terminal 3´-OH of a DNA initiator accompanied by the release of inorganic phosphate.


  • Single-stranded DNA is preferred as an initiator
  • Polymerisation is not template-dependent
  • Addition of 1 mM Co2+ (as CoCl2) in the reaction buffer allows the tailing of 3´ ends with varying degrees of efficiency
  • Source: Recombinant E. coli strain


Applications:
Tailing reactions to add complementary homopolymer tails to DNA vectors and cDNA.
3´ end-labeling.
TUNEL assays.


5X Reaction buffer: 500 mM cacodylate buffer (pH 6,8 at 25 °C), 5 mM CoCl2, 0,5 mM DTT.


Fornecimento: Kit includes terminal deoxynucleotidyl transferase, recombinant 30 U/µl and terminal transferase 5X buffer.

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