Recombinant terminal deoxynucleotidyl transferase
Fornecedor: Promega
Terminal deoxynucleotidyl transferase, recombinant, catalyses the repetitive addition of mononucleotides to the terminal 3´-OH of a DNA initiator accompanied by the release of inorganic phosphate.
- Single-stranded DNA is preferred as an initiator
- Polymerisation is not template-dependent
- Addition of 1 mM Co2+ (as CoCl2) in the reaction buffer allows the tailing of 3´ ends with varying degrees of efficiency
- Source: Recombinant E. coli strain
Applications:
Tailing reactions to add complementary homopolymer tails to DNA vectors and cDNA.
3´ end-labeling.
TUNEL assays.
5X Reaction buffer: 500 mM cacodylate buffer (pH 6,8 at 25 °C), 5 mM CoCl2, 0,5 mM DTT.
Fornecimento: Kit includes terminal deoxynucleotidyl transferase, recombinant 30 U/µl and terminal transferase 5X buffer.
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