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Oxidative stress assays, GSH/GSSG-Glo™ assay

Fornecedor: Promega

V6611 V6612
PROMV6611EA 751 EUR
PROMV6611 PROMV6612
Oxidative stress assays, GSH/GSSG-Glo™ assay
Ensaios Ensaios celulares

The GSH/GSSG-Glo™ Assay is a luminescence-based system for the detection and quantification of total glutathione (GSH +GSSG), GSSG and GSH/GSSG ratios in cultured cells. A change in GSH levels is important in the assessment of toxicological responses and is an indicator of oxidative stress, potentially leading to apoptosis or cell death. The assay provides a simple, rapid multiwell-plate format where stable luminescent signals are correlated with either the total GSH or the GSSG concentration of a sample directly in culture wells.


  • Measures physiologically relevant GSH/GSSG ratios
  • Simple protocol minimises sample handling, reducing variability


The assay generates values for total glutathione and GSSG. Since the value for total glutathione represents the signals from both the reduced and oxidised glutathione, subtracting the GSSG reaction signal from the total glutathione signal yields the value of reduced glutathione in the sample.


Fornecimento: The complete assay kit includes Passive Lysis Buffer (5X), DTT, Glutathione Reaction Buffer, NEM (25 mM), Luciferin-NT, Glutathione S-Transferase, Glutathione (5 mM), Luciferin Detection Reagent and Reconstitution Buffer with esterase.

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