Luminescent kinase assay, Kinase-Glo®
Fornecedor: Promega
The Kinase-Glo® Luminescent Kinase Assays are homogeneous non-radioactive methods for determining the activity of purified kinases by quantifying the amount of ATP remaining in solution following a kinase reaction. Kinase-Glo® Assay is linear to 10 μm ATP.
- Simple
- Scalable
- Easy to automate
Kinase-Glo® Luminescent Kinase Assays are designed for use with multiwell plate formats, making them ideal for automated high-throughput screening (HTS), and they can be used to assay protein, lipid and sugar kinases. The assay procedure involves addition of a single reagent directly to a completed kinase reaction. This addition results in the generation of a luminescent signal correlated with the amount of ATP present and inversely proportional to the amount of kinase activity.
Fornecimento: Kit includes Kinase-Glo® buffer and Kinase-Glo® substrate.
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