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Restriction enzymes, PvuI

Fornecedor: Promega

R6321 R6325
PROMR63210100EA 120 EUR
PROMR63210100 PROMR63250500
Restriction enzymes, PvuI
Reagentes de ácidos nucleicos Restriction Enzymes

These PvuI restriction enzyme recognises the cut site of CG AT˅CG and GC˄TA GC. Ends generated by PvuI can be directly ligated to ends generated by SgfI. The PvuI site is regenerated in the ligation product, but the SgfI site is not.


  • Relegation of DNA fragments with dam methylation is difficult
  • Active and capable of digestion directly in GoTaq® Green Master Mix
  • Incubation with buffer D at 37 °C
  • Source: Proteus vulgaris


Storage Buffer: 10 mM Tris-HCl (pH 7,3 @ 25 °C), 50 mM NaCl, 0,1% Triton® X-100, 1 mM DTT, 0,1 mM EDTA, 0,5 mg/ml BSA and 50% glycerol.


Fornecimento: The kit includes 1×1 ml buffer D 10X, 1×150 µl BSA acetylated with concentration of 10 mg/ml and 1×100 U PvuI.

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