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cAMP assay, GloSensor™

Fornecedor: Promega

E1290 E1291 E2301
PROME1290EA 588 EUR
PROME1290 PROME1291 PROME2301
cAMP assay, GloSensor™
Ensaios Ensaios celulares

The GloSensor™ cAMP assay provides an extremely sensitive and easy to use format for the interrogation of overexpressed or endogenous GPCRs that signal via changes in the intracellular concentration of cAMP.


  • Suitable for HTS/uHTS
  • Live-cell, non-lytic assay format
  • Simple, scalable assay protocol
  • High Z´ and large signal:background ratio


The assay uses genetically encoded biosensor variants with cAMP binding domains fused to mutant forms of Photinus pyralis luciferase. Upon binding to cAMP, conformational changes occur that promote large increases in light output. Following pre-equilibration with substrate, cells transiently or stably expressing a biosensor variant can be used to assay GPCR function using a live-cell, non-lytic assay format, enabling facile kinetic measurements of cAMP accumulation or turnover in living cells. Moreover, the assay offers a broad dynamic range, showing up to 500-fold changes in light output. Extreme sensitivity allows detection of Gi-coupled receptor activation or inverse agonist activity in the absence of artificial stimulation by compounds such as forskolin.

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