RNA polymerase promoter sequencing primers, Promega
Fornecedor: Promega
The SP6 and T7 promoter primers are designed for sequencing inserts cloned into the pGEM® Vectors.
- Single-stranded
- Supplied at a concentration of 10 µg/ml
The SP6 promoter primer for sequencing inserts cloned into the pGEM®, pALTER®-MAX and pCI-neo vectors is designed to be annealed to single-stranded DNA or, after alkaline denaturation, to double-stranded DNA. The promoter primers are purified by gel electrophoresis or HPLC.
The T7 EEV promoter primer is suitable for sequencing the pALTER®-MAX, pCMVTnT™, pTnT™ and phMGFP vectors, and the pCI/pSI series of mammalian expression vectors. T7 promoter sites flanking the multiple cloning region of all pGEM® vectors.
Primer sequences:
SP6: 5´-d(TATTTAGGTGACACTATAG)-3´
T7: 5´-d(TAATACGACTCACTATAGGG)-3´
T7 EEV: 5´-d(AAGGCTAGAGTACTTAATACGA)-3´
Storage: –20 °C
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