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RNA polymerase promoter sequencing primers, Promega

Fornecedor: Promega

Q5011 Q5021 Q5741 Q6700
PROMQ50110002EA 138 EUR
PROMQ50110002 PROMQ50210002 PROMQ57410002 PROMQ67000002
RNA polymerase promoter sequencing primers, Promega
Reagentes de ácidos nucleicos Reagentes de Sequenciação

The SP6 and T7 promoter primers are designed for sequencing inserts cloned into the pGEM® Vectors.


  • Single-stranded
  • Supplied at a concentration of 10 µg/ml


The SP6 promoter primer for sequencing inserts cloned into the pGEM®, pALTER®-MAX and pCI-neo vectors is designed to be annealed to single-stranded DNA or, after alkaline denaturation, to double-stranded DNA. The promoter primers are purified by gel electrophoresis or HPLC.


The T7 EEV promoter primer is suitable for sequencing the pALTER®-MAX, pCMVTnT™, pTnT™ and phMGFP vectors, and the pCI/pSI series of mammalian expression vectors. T7 promoter sites flanking the multiple cloning region of all pGEM® vectors.


Primer sequences:
SP6: 5´-d(TATTTAGGTGACACTATAG)-3´
T7: 5´-d(TAATACGACTCACTATAGGG)-3´
T7 EEV: 5´-d(AAGGCTAGAGTACTTAATACGA)-3´


Storage: –20 °C

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