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T4 DNA polymerase

Fornecedor: Promega

M4211 M4215
PROMM42110100EA 121 EUR
PROMM42110100 PROMM42150500
T4 DNA polymerase
Reagentes de ácidos nucleicos Nucleic Acid Modification and Labelling

T4 DNA Polymerase catalyses the 5´→3´ synthesis of DNA from a primed single-stranded DNA template.


  • High fidelity enzyme of choice for applications where misincorporation is a concern
  • Flexible polymerase used in a variety of molecular applications
  • Heat inactivated by heating at 75 °C for 10 minutes
  • Source: Recombinant E. coli strain


Although possessing a potent 3´→5´ proofreading exonuclease, T4 DNA Polymerase contains no 5´→3´ exonuclease activity. T4 DNA Polymerase can be used to fill in 5´ protruding ends with labeled or unlabeled dNTPs or for the generation of blunt ends from DNA molecules with 3´ overhangs or in vitro mutagenesis.


Storage buffer: 200 mM potassium phosphate (pH 6,5 at 25 °C), 2 mM DTT and 50% glycerol.


Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 55 nmol of dTTP into acid-precipitable material in 30 minutes at 39 °C using poly(dA):oligo(dT) as a substrate.


Fornecimento: Contains T4 DNA Polymerase (5 - 10 U/µl) and 10X Reaction Buffer (250 mM Tris-acetate, pH 7,7; 1 M potassium acetate; 100 mM magnesium acetate; and 10 mM DTT).

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