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Master mixes, GoTaq® Hot Start master mix

Fornecedor: Promega

M5122 M5123 M5132 M5133
PROMM5122EA 257 EUR
PROMM5122 PROMM5123 PROMM5132 PROMM5133
Master mixes, GoTaq® Hot Start master mix
Reagentes de ácidos nucleicos End-point PCR Enzymes and Kits

GoTaq® Hot Start Master Mix is a premixed ready-to-use solution containing GoTaq® Hot Start Polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR.


  • All-in-one master mix: Just add template and primers
  • Eliminate nonspecific amplification with a hot start enzyme
  • Green master mix for direct-to-gel analysis
  • Use colourless master mix when post-amplification fluorescence or absorbance analysis is required
  • Reactions can be set up in less than a minute; simply add your template, water and primers


GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 94 to 95 °C for two minutes. This enables hot-start PCR, where polymerase activity is eliminated or minimized at temperatures below 70 °C. GoTaq® Hot Start Polymerase exhibits 5´→3´exonuclease activity.


GoTaq® Hot Start Green Master Mix also contains two dyes (yellow and blue) that separate to allow easy monitoring during electrophoresis.


Fornecimento: Kit includes 2X of GoTaq® Hot Start Colourless Master Mix or GoTaq® Hot Start Green Master Mix and nuclease-free water.

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