Master mixes, GoTaq®
Fornecedor: Promega
GoTaq® Master Mixes are ready-to-use 2X solutions that contain GoTaq® DNA Polymerase, dNTPs, MgCl₂ and reaction buffer at optimal concentrations for efficient amplification of DNA templates by PCR.
- Directly substitute into your current PCR conditions
- Green master mix for direct-to-gel analysis after amplification
- Use colourless master mix for post-amplification analysis by fluorescence or absorbance without prior DNA purification
- Reactions can be set up in less than a minute - simply add template, water and primers
GoTaq® DNA polymerase is a proprietary formulation of Taq DNA polymerase that gives robust amplification equal to and, in some cases, superior to that of standard Taq DNA polymerase.
GoTaq® Green Master Mix includes two dyes (blue and yellow) that allow monitoring of progress during electrophoresis. The blue dye comigrates at the same rate as 3 to 5 kb DNA fragments in a 1% agarose gel. The yellow dye migrates ahead of primers (<50 bp). GoTaq® Colorless Master Mix has the same formulation as the GoTaq® Green Master Mix but does not include the dyes.
Fornecimento: Kit includes 2X of GoTaq® Colourless master mix or GoTaq® Green master mix and nuclease-free water.
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