Master mixes, GoTaq® G2 Hot Start Master Mix
Fornecedor: Promega
GoTaq® G2 Hot Start Master Mix is a premixed ready-to-use solution containing GoTaq® G2 Hot Start Polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR.
- All-in-one master mix: Just add template and primers
- Eliminate non specific amplification with a hot-start enzyme
- Green master mix contains a gel loading dye for direct-to-gel analysis
- Use colourless master mix when post-amplification fluorescence or absorbance analysis is required
GoTaq® G2 Hot Start Polymerase is bound to a proprietary antibody that blocks activity at temperatures below 70 °C for convenient, room temperature reaction set-up. Polymerase activity is restored during the initial denaturation step, when amplification reactions are heated at 94 to 95 °C for two minutes, allowing hot-start PCR following room-temperature reaction assembly. Hot-start PCR is advantageous for some amplification targets because it may eliminate or minimise primer-dimer and non specific products. In some cases, hot-start PCR may improve yields. GoTaq® G2 Hot Start Polymerase also exhibits 5´→3´ exonuclease activity.
Fornecimento: Kit includes 2X of GoTaq® G2 Hot Start Colourless Master Mix or GoTaq® G2 Hot Start Green Master Mix and nuclease-free water.
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