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GoTaq® G2 hot start DNA polymerase

Fornecedor: Promega

M7401 M7405 M7406 M7408
PROMM7401EA 85.1 EUR
PROMM7401 PROMM7405 PROMM7406 PROMM7408
GoTaq® G2 hot start DNA polymerase
Reagentes de ácidos nucleicos End-point PCR Enzymes and Kits

High performance GoTaq® G2 DNA Polymerase is bound to a proprietary antibody that blocks polymerase activity. Polymerase activity is restored during the initial denaturation step, when amplification reactions are heated at 94 to 95°C for two minutes, allowing hot-start PCR in which polymerase activity is inhibited at temperatures below 70 °C for convenient, room-temperature reaction set-up.


  • Eliminate non specific amplification with a hot-start enzyme
  • Detect lower-copy-number targets
  • Optimise reaction conditions with a Flexi Reaction Buffer and separate MgCl₂
  • Supplied at a concentration of 5 U/μl


Hot-start PCR is advantageous for some amplification targets because it may eliminate or minimise primer-dimer and non specific products. In some cases, hot-start PCR may improve yields. GoTaq® G2 Hot Start Polymerase exhibits 5 →3 exonuclease activity.


Fornecimento: Kit includes GoTaq® G2 Hot Start Polymerase (5 U/µl), 5X Green GoTaq® Flexi Buffer, 5X Colorless GoTaq® Flexi Buffer, and a separate vial of 25 mM MgCl2.

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