GoTaq® G2 hot start DNA polymerase
Fornecedor: Promega
High performance GoTaq® G2 DNA Polymerase is bound to a proprietary antibody that blocks polymerase activity. Polymerase activity is restored during the initial denaturation step, when amplification reactions are heated at 94 to 95°C for two minutes, allowing hot-start PCR in which polymerase activity is inhibited at temperatures below 70 °C for convenient, room-temperature reaction set-up.
- Eliminate non specific amplification with a hot-start enzyme
- Detect lower-copy-number targets
- Optimise reaction conditions with a Flexi Reaction Buffer and separate MgCl₂
- Supplied at a concentration of 5 U/μl
Hot-start PCR is advantageous for some amplification targets because it may eliminate or minimise primer-dimer and non specific products. In some cases, hot-start PCR may improve yields. GoTaq® G2 Hot Start Polymerase exhibits 5 →3 exonuclease activity.
Fornecimento: Kit includes GoTaq® G2 Hot Start Polymerase (5 U/µl), 5X Green GoTaq® Flexi Buffer, 5X Colorless GoTaq® Flexi Buffer, and a separate vial of 25 mM MgCl2.
Learn more

About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...