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GoTaq® Hot Start DNA polymerase

Fornecedor: Promega

M5001 M5005 M5006
PROMM5001EA 123 EUR
PROMM5001 PROMM5005 PROMM5006
GoTaq® Hot Start DNA polymerase
Reagentes de ácidos nucleicos End-point PCR Enzymes and Kits

GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity.


  • Minimise non specific amplification and primer-dimers
  • Direct-to-gel convenience


The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 94 to 95 °C for two minutes. This enables hot start PCR, where polymerase activity is eliminated or minimised at temperatures below 70 °C. GoTaq® Hot Start Polymerase also exhibits 5´→3´ exonuclease activity. The enzyme is supplied with a tube of 25 mM MgCl₂ to optimise the magnesium concentration.


Fornecimento: Kit includes GoTaq® Hot Start Polymerase (5 U/µl), 5X Green GoTaq® Flexi Buffer, 5X Colourless GoTaq® Flexi Buffer, and a separate vial of 25 mM MgCl₂.

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