GoTaq® Hot Start DNA polymerase
Fornecedor: Promega
GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity.
- Minimise non specific amplification and primer-dimers
- Direct-to-gel convenience
The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 94 to 95 °C for two minutes. This enables hot start PCR, where polymerase activity is eliminated or minimised at temperatures below 70 °C. GoTaq® Hot Start Polymerase also exhibits 5´→3´ exonuclease activity. The enzyme is supplied with a tube of 25 mM MgCl₂ to optimise the magnesium concentration.
Fornecimento: Kit includes GoTaq® Hot Start Polymerase (5 U/µl), 5X Green GoTaq® Flexi Buffer, 5X Colourless GoTaq® Flexi Buffer, and a separate vial of 25 mM MgCl₂.
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